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Two parallel pathways are required for ultrasound-evoked behavioral changes in Caenorhabditis elegans

Uri Magaram, Connor Weiss, Aditya Vasan, Kirthi C. Reddy, James Friend, Sreekanth H. Chalasani
doi: https://doi.org/10.1101/2021.10.29.466533
Uri Magaram
1Neurosciences Graduate Program, University of California San Diego, La Jolla, CA 92093
2Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037
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Connor Weiss
2Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037
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Aditya Vasan
3Medically Advanced Devices Laboratory, Department of Mechanical and Aerospace Engineering, Jacobs School of Engineering and Department of Surgery, School of Medicine, University of California San Diego, La Jolla, CA 92093
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Kirthi C. Reddy
2Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037
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James Friend
3Medically Advanced Devices Laboratory, Department of Mechanical and Aerospace Engineering, Jacobs School of Engineering and Department of Surgery, School of Medicine, University of California San Diego, La Jolla, CA 92093
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Sreekanth H. Chalasani
2Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037
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  • For correspondence: schalasani@salk.edu
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Abstract

Ultrasound has been shown to affect the function of both neurons and non-neuronal cells. However, the underlying molecular machinery has been poorly understood. Here, we show that at least two mechanosensitive proteins act in parallel to generate C. elegans behavioral responses to ultrasound stimuli. We first show that these animals generate reversals in response to a single 10 msec pulse from a 2.25 MHz ultrasound transducer. Next, we show that the pore-forming subunit of the mechanosensitive channel TRP-4, and a DEG/ENaC/ASIC ion channel MEC-4, are both required for this ultrasound-evoked reversal response. Further, the trp-4 mec-4 double mutant shows a stronger behavioral deficit compared to either single mutant. Finally, overexpressing TRP-4 in specific chemosensory neurons can rescue the ultrasound-triggered behavioral deficit in the mec-4 null mutant, suggesting that these two pathways act in parallel. Together, we demonstrate that multiple mechanosensitive proteins likely cooperate to transform ultrasound stimuli into behavioral changes.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted November 02, 2021.
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Two parallel pathways are required for ultrasound-evoked behavioral changes in Caenorhabditis elegans
Uri Magaram, Connor Weiss, Aditya Vasan, Kirthi C. Reddy, James Friend, Sreekanth H. Chalasani
bioRxiv 2021.10.29.466533; doi: https://doi.org/10.1101/2021.10.29.466533
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Two parallel pathways are required for ultrasound-evoked behavioral changes in Caenorhabditis elegans
Uri Magaram, Connor Weiss, Aditya Vasan, Kirthi C. Reddy, James Friend, Sreekanth H. Chalasani
bioRxiv 2021.10.29.466533; doi: https://doi.org/10.1101/2021.10.29.466533

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