Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Multi-area recordings and optogenetics in the awake, behaving marmoset

View ORCID ProfilePatrick Jendritza, Frederike J. Klein, View ORCID ProfilePascal Fries
doi: https://doi.org/10.1101/2021.10.30.466578
Patrick Jendritza
1Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Frankfurt, Germany
2International Max Planck Research School for Neural Circuits, Frankfurt, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Patrick Jendritza
  • For correspondence: patrick.jendritza@esi-frankfurt.de
Frederike J. Klein
1Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Frankfurt, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pascal Fries
1Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Frankfurt, Germany
2International Max Planck Research School for Neural Circuits, Frankfurt, Germany
3Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Pascal Fries
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Data/Code
  • Preview PDF
Loading

Abstract

The common marmoset has emerged as a key primate model in neuroscience. Marmosets are small in size, show great potential as transgenic models and exhibit complex behaviors. These advantages place the marmoset model in the critical gap between rodents and larger primates. Thus, it is necessary to develop technology that enables monitoring and manipulation of the neural circuits underlying the behavior of the marmoset. Here, we present a novel approach to record and optogenetically manipulate neural activity in the awake, behaving marmoset. Our design utilizes a light-weight, 3D printed titanium chamber that can house several high-density silicon probes for semi-chronic recordings, while enabling simultaneous optogenetic stimulation. Surgical procedures are streamlined via custom 3D printed guides and implantation holders. We demonstrate the application of our method by recording multi- and single-unit data from areas V1 and V6 with 192 channels simultaneously, and show for the first time that optogenetic activation of excitatory neurons in area V6 can influence behavior in a detection task. Together, the work presented here will support future studies investigating the neural basis of perception and behavior in the marmoset.

Competing Interest Statement

P.F. has a patent on thin-film electrodes and is beneficiary of a respective license contract with Blackrock Microsystems LLC (Salt Lake City, UT, USA). P.F. is a member of the Scientific Technical Advisory Board of CorTec GmbH (Freiburg, Germany) and is managing director of Brain Science GmbH (Frankfurt am Main, Germany).

Footnotes

  • https://github.com/PJendritza/Marmo

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted November 02, 2021.
Download PDF
Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Multi-area recordings and optogenetics in the awake, behaving marmoset
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Multi-area recordings and optogenetics in the awake, behaving marmoset
Patrick Jendritza, Frederike J. Klein, Pascal Fries
bioRxiv 2021.10.30.466578; doi: https://doi.org/10.1101/2021.10.30.466578
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Multi-area recordings and optogenetics in the awake, behaving marmoset
Patrick Jendritza, Frederike J. Klein, Pascal Fries
bioRxiv 2021.10.30.466578; doi: https://doi.org/10.1101/2021.10.30.466578

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3605)
  • Biochemistry (7575)
  • Bioengineering (5529)
  • Bioinformatics (20806)
  • Biophysics (10333)
  • Cancer Biology (7986)
  • Cell Biology (11644)
  • Clinical Trials (138)
  • Developmental Biology (6610)
  • Ecology (10214)
  • Epidemiology (2065)
  • Evolutionary Biology (13622)
  • Genetics (9543)
  • Genomics (12851)
  • Immunology (7923)
  • Microbiology (19550)
  • Molecular Biology (7666)
  • Neuroscience (42125)
  • Paleontology (308)
  • Pathology (1258)
  • Pharmacology and Toxicology (2203)
  • Physiology (3268)
  • Plant Biology (7044)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1951)
  • Systems Biology (5427)
  • Zoology (1118)