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Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for IFT of radial spokes

View ORCID ProfileKarl Lechtreck, Yi Liu, Jin Dai, Rama A. Alkhofash, Jack Butler, Lea Alford, View ORCID ProfilePinfen Yang
doi: https://doi.org/10.1101/2021.10.31.466660
Karl Lechtreck
1Department of Cellular Biology, University of Georgia, Athens, GA 30602
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  • For correspondence: lechtrek@uga.edu pinfen.yang@marquette.edu
Yi Liu
2Department of Biological Sciences, Marquette University, Milwaukee, WI 53233
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Jin Dai
1Department of Cellular Biology, University of Georgia, Athens, GA 30602
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Rama A. Alkhofash
1Department of Cellular Biology, University of Georgia, Athens, GA 30602
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Jack Butler
1Department of Cellular Biology, University of Georgia, Athens, GA 30602
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Lea Alford
3Division of Natural Sciences, Oglethorpe University, Atlanta, GA 30319
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Pinfen Yang
2Department of Biological Sciences, Marquette University, Milwaukee, WI 53233
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  • For correspondence: lechtrek@uga.edu pinfen.yang@marquette.edu
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Abstract

Intraflagellar transport (IFT) carries proteins into flagella but how IFT trains interact with the large number of diverse proteins required to assemble flagella remains largely unknown. Here, we show that IFT of radial spokes in Chlamydomonas requires ARMC2/PF27, a conserved armadillo repeat protein associated with male infertility and reduced lung function. Chlamydomonas ARMC2 was highly enriched in growing flagella and tagged ARMC2 and the spoke protein RSP3 comigrated on anterograde trains. In contrast, a cargo and an adapter of inner and outer dynein arms moved independently of ARMC2, indicating that unrelated cargoes distribute stochastically onto the IFT trains. After concomitant unloading at the flagellar tip, RSP3 attached to the axoneme whereas ARMC2 diffused back to the cell body. In armc2/pf27 mutants, IFT of radial spokes was abolished and the presence of radial spokes was limited to the proximal region of flagella. We conclude that ARMC2 is a cargo adapter required for IFT of radial spokes to ensure their assembly along flagella. ARMC2 belongs to a growing class of cargo-specific adapters that enable flagellar transport of preassembled axonemal substructures by IFT.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵@ co-first authors

  • Abbreviations

    CHX
    cycloheximide
    FP
    fluorescent protein
    IFT
    intraflagellar transport
    NG
    mNeonGreen
    mS
    mScarlet-I
    RS
    radial spoke.
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    Posted October 31, 2021.
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    Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for IFT of radial spokes
    Karl Lechtreck, Yi Liu, Jin Dai, Rama A. Alkhofash, Jack Butler, Lea Alford, Pinfen Yang
    bioRxiv 2021.10.31.466660; doi: https://doi.org/10.1101/2021.10.31.466660
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    Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for IFT of radial spokes
    Karl Lechtreck, Yi Liu, Jin Dai, Rama A. Alkhofash, Jack Butler, Lea Alford, Pinfen Yang
    bioRxiv 2021.10.31.466660; doi: https://doi.org/10.1101/2021.10.31.466660

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