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Reciprocal zebrafish-medaka hybrids reveal maternal control of zygotic genome activation timing

Krista R. Gert, Luis Enrique Cabrera Quio, Maria Novatchkova, Yixuan Guo, View ORCID ProfileBradley R. Cairns, View ORCID ProfileAndrea Pauli
doi: https://doi.org/10.1101/2021.11.03.467109
Krista R. Gert
1Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
2Vienna BioCenter PhD Program, a Doctoral School of the University at Vienna and the Medical University of Vienna, 1030 Vienna, Austria
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Luis Enrique Cabrera Quio
1Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
2Vienna BioCenter PhD Program, a Doctoral School of the University at Vienna and the Medical University of Vienna, 1030 Vienna, Austria
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Maria Novatchkova
1Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
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Yixuan Guo
3Howard Hughes Medical Institute, Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
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Bradley R. Cairns
3Howard Hughes Medical Institute, Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
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  • ORCID record for Bradley R. Cairns
Andrea Pauli
1Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
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  • For correspondence: andrea.pauli@imp.ac.at
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Abstract

After fertilization, the sperm and egg contribute unequally to the newly formed zygote. While the sperm contributes mainly paternal DNA, the egg provides both maternal DNA and the bulk of the future embryonic cytoplasm. Most embryonic processes (like the onset of zygotic transcription) depend on maternally-provided cytoplasmic components, and it is largely unclear whether paternal components besides the centrosome play a role in the regulation of early embryogenesis. Here we report a reciprocal zebrafish-medaka hybrid system as a powerful tool to investigate paternal vs. maternal influence during early development. By combining expression of zebrafish Bouncer on the medaka egg with artificial egg activation, we demonstrate the in vitro generation of paternal zebrafish x maternal medaka (reripes) hybrids. These hybrids complement the previously established paternal medaka x maternal zebrafish (latio) hybrids (Herberg et al., 2018). As proof of concept, we investigated maternal vs. paternal control of zygotic genome activation (ZGA) timing using this reciprocal hybrid system. RNA-seq analysis of the purebred fish species and hybrids revealed that the onset of ZGA is primarily governed by the egg. Overall, our study establishes the reciprocal zebrafish-medaka hybrid system as a versatile tool to dissect parental control mechanisms during early development.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted November 04, 2021.
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Reciprocal zebrafish-medaka hybrids reveal maternal control of zygotic genome activation timing
Krista R. Gert, Luis Enrique Cabrera Quio, Maria Novatchkova, Yixuan Guo, Bradley R. Cairns, Andrea Pauli
bioRxiv 2021.11.03.467109; doi: https://doi.org/10.1101/2021.11.03.467109
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Reciprocal zebrafish-medaka hybrids reveal maternal control of zygotic genome activation timing
Krista R. Gert, Luis Enrique Cabrera Quio, Maria Novatchkova, Yixuan Guo, Bradley R. Cairns, Andrea Pauli
bioRxiv 2021.11.03.467109; doi: https://doi.org/10.1101/2021.11.03.467109

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