Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Going through phages: A Computational approach to Revealing the role of prophage in Staphylococcus aureus

View ORCID ProfileTyrome Sweet, Suzanne Sindi, View ORCID ProfileMark Sistrom
doi: https://doi.org/10.1101/2021.11.10.468171
Tyrome Sweet
aDepartment of Life and Environmental Sciences, University of California, Merced, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tyrome Sweet
  • For correspondence: tsweet@ucmerced.edu
Suzanne Sindi
bDepartment of Applied Mathematics, University of California, Merced, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mark Sistrom
aDepartment of Life and Environmental Sciences, University of California, Merced, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mark Sistrom
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Prophages have important roles in virulence, antibiotic resistance and genome evolution in Staphylococcus aureus. Rapid growth in the number of sequenced S. aureus genomes allows for an investigation of prophage sequences in S. aureus at an unprecedented scale. We developed a computational pipeline to detect and analyze prophage sequences in nearly 10,011 S. aureus genomes, discovering thousands of putative prophage sequences with genes encoding virulence factors and antibiotic resistance.

Importance Bacteriophages (phages) play key roles in bacterial evolution, governing abundance, adaptation and diversity of bacterial communities. Temperate phage can facilitate bacterial adaptation via transduction of novel genes. This study takes advantage of the unprecedented quantity of genomic sequencing in public repositories to analyze viral genes in 10,000 Staphylococcus aureus genomes. We found 196,727 prophage sequences, with an estimated total of 129,935 genes. We determined the function of these genes, identifying a large quantity of novel genes that benefit the host such as beta-lactamase, enterotoxins and cytotoxins. These results will inform studies of bacterial evolution and adaptation, by identifying the mechanism of horizontal transfer of genes that confer adaptive traits to bacteria, especially in the context of antibiotic resistance.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted November 12, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Going through phages: A Computational approach to Revealing the role of prophage in Staphylococcus aureus
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Going through phages: A Computational approach to Revealing the role of prophage in Staphylococcus aureus
Tyrome Sweet, Suzanne Sindi, Mark Sistrom
bioRxiv 2021.11.10.468171; doi: https://doi.org/10.1101/2021.11.10.468171
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Going through phages: A Computational approach to Revealing the role of prophage in Staphylococcus aureus
Tyrome Sweet, Suzanne Sindi, Mark Sistrom
bioRxiv 2021.11.10.468171; doi: https://doi.org/10.1101/2021.11.10.468171

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4113)
  • Biochemistry (8815)
  • Bioengineering (6518)
  • Bioinformatics (23459)
  • Biophysics (11789)
  • Cancer Biology (9207)
  • Cell Biology (13322)
  • Clinical Trials (138)
  • Developmental Biology (7436)
  • Ecology (11409)
  • Epidemiology (2066)
  • Evolutionary Biology (15149)
  • Genetics (10436)
  • Genomics (14043)
  • Immunology (9171)
  • Microbiology (22153)
  • Molecular Biology (8812)
  • Neuroscience (47566)
  • Paleontology (350)
  • Pathology (1428)
  • Pharmacology and Toxicology (2491)
  • Physiology (3730)
  • Plant Biology (8079)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2220)
  • Systems Biology (6037)
  • Zoology (1253)