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Functionalizing silica sol-gel with entrapped plant virus-based immunosorbent nanoparticles

View ORCID ProfileMatthew J. McNulty, Naomi Hamada, Jesse Delzio, Liber McKee, View ORCID ProfileSomen Nandi, Marjorie L. Longo, View ORCID ProfileKaren A. McDonald
doi: https://doi.org/10.1101/2021.11.12.468100
Matthew J. McNulty
1Department of Chemical Engineering, University of California, Davis, CA, USA
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Naomi Hamada
1Department of Chemical Engineering, University of California, Davis, CA, USA
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Jesse Delzio
1Department of Chemical Engineering, University of California, Davis, CA, USA
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Liber McKee
1Department of Chemical Engineering, University of California, Davis, CA, USA
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Somen Nandi
1Department of Chemical Engineering, University of California, Davis, CA, USA
2Global HealthShare® Initiative, University of California, Davis, CA, USA
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Marjorie L. Longo
1Department of Chemical Engineering, University of California, Davis, CA, USA
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Karen A. McDonald
1Department of Chemical Engineering, University of California, Davis, CA, USA
2Global HealthShare® Initiative, University of California, Davis, CA, USA
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  • For correspondence: kamcdonald@ucdavis.edu
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Abstract

Advancements in understanding and engineering of virus-based nanomaterials (VBNs) for biomedical applications motivate a need to explore the interfaces between VBNs and other biomedically-relevant chemistries and materials. While several strategies have been used to investigate some of these interfaces with promising initial results, including VBN-containing slow-release implants and VBN-activated bioceramic bone scaffolds, there remains a need to establish VBN-immobilized three dimensional materials that exhibit improved stability and diffusion characteristics for biosensing and other analyte-capture applications. Silica sol-gel chemistries have been researched for biomedical applications over several decades and are well understood; various cellular organisms and biomolecules (e.g., bacteria, algae, enzymes) have been immobilized in silica sol-gels to improve viability, activity, and form factor (i.e., ease of use). Here we present the immobilization of an antibody-binding VBN in silica sol-gel by pore confinement. We have shown that the resulting system is sufficiently diffuse to allow antibodies to migrate in and out of the matrix. We also show that the immobilized VBN is capable of antibody binding and elution functionality under different buffer conditions for multiple use cycles. The promising results of the VBN and silica sol-gel interface indicate a general applicability for VBN-based bioseparations and biosensing applications.

Competing Interest Statement

The authors have declared no competing interest.

  • Abbreviations

    Cy5-TMV
    Cyanine 5 conjugated TMV
    hIgG
    human immunoglobulin G
    PEG
    polyethylene glycol
    PLGA
    poly(lactic-co-glycolic acid)
    TMOS
    tetramethyl orthosilicate
    TMV
    tobacco mosaic virus
    TVCV
    turnip vein clearing virus.
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    Posted November 12, 2021.
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    Functionalizing silica sol-gel with entrapped plant virus-based immunosorbent nanoparticles
    Matthew J. McNulty, Naomi Hamada, Jesse Delzio, Liber McKee, Somen Nandi, Marjorie L. Longo, Karen A. McDonald
    bioRxiv 2021.11.12.468100; doi: https://doi.org/10.1101/2021.11.12.468100
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    Functionalizing silica sol-gel with entrapped plant virus-based immunosorbent nanoparticles
    Matthew J. McNulty, Naomi Hamada, Jesse Delzio, Liber McKee, Somen Nandi, Marjorie L. Longo, Karen A. McDonald
    bioRxiv 2021.11.12.468100; doi: https://doi.org/10.1101/2021.11.12.468100

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