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Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cells

Beau R. Webber, Matthew J. Johnson, Nicholas J. Slipek, Walker S. Lahr, Anthony P. DeFeo, Joseph G. Skeate, Xiaohong Qiu, Blaine Rathmann, Miechaleen D. Diers, Bryce Wick, Tom Henley, Modassir Choudhry, R. Scott McIvor, Branden S. Moriarity
doi: https://doi.org/10.1101/2021.11.12.468427
Beau R. Webber
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Matthew J. Johnson
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Nicholas J. Slipek
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Walker S. Lahr
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Anthony P. DeFeo
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Joseph G. Skeate
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Xiaohong Qiu
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Blaine Rathmann
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Miechaleen D. Diers
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Bryce Wick
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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Tom Henley
4Intima Bioscience, New York, USA
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Modassir Choudhry
4Intima Bioscience, New York, USA
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R. Scott McIvor
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
5Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
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Branden S. Moriarity
1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
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  • For correspondence: mori0164@umn.edu
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Article Information

doi 
https://doi.org/10.1101/2021.11.12.468427
History 
  • November 13, 2021.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Beau R. Webber1,2,3,*,
  2. Matthew J. Johnson1,2,3,*,
  3. Nicholas J. Slipek1,2,3,
  4. Walker S. Lahr1,2,3,
  5. Anthony P. DeFeo1,2,3,
  6. Joseph G. Skeate1,2,3,
  7. Xiaohong Qiu1,2,3,
  8. Blaine Rathmann1,2,3,
  9. Miechaleen D. Diers1,2,3,
  10. Bryce Wick1,2,3,
  11. Tom Henley4,
  12. Modassir Choudhry4,
  13. R. Scott McIvor3,5 and
  14. Branden S. Moriarity1,2,3,*
  1. 1Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA
  2. 2Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA
  3. 3Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA
  4. 4Intima Bioscience, New York, USA
  5. 5Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
  1. ↵*Corresponding author; email: mori0164{at}umn.edu
  1. ↵* These authors contributed equally.

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Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cells
Beau R. Webber, Matthew J. Johnson, Nicholas J. Slipek, Walker S. Lahr, Anthony P. DeFeo, Joseph G. Skeate, Xiaohong Qiu, Blaine Rathmann, Miechaleen D. Diers, Bryce Wick, Tom Henley, Modassir Choudhry, R. Scott McIvor, Branden S. Moriarity
bioRxiv 2021.11.12.468427; doi: https://doi.org/10.1101/2021.11.12.468427
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Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cells
Beau R. Webber, Matthew J. Johnson, Nicholas J. Slipek, Walker S. Lahr, Anthony P. DeFeo, Joseph G. Skeate, Xiaohong Qiu, Blaine Rathmann, Miechaleen D. Diers, Bryce Wick, Tom Henley, Modassir Choudhry, R. Scott McIvor, Branden S. Moriarity
bioRxiv 2021.11.12.468427; doi: https://doi.org/10.1101/2021.11.12.468427

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