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Optogenetic induction of appetitive and aversive taste memories in Drosophila

Meghan Jelen, View ORCID ProfilePierre-Yves Musso, View ORCID ProfilePierre Junca, View ORCID ProfileMichael D. Gordon
doi: https://doi.org/10.1101/2021.11.12.468444
Meghan Jelen
1Department of Zoology and Life Sciences Institute, University of British Columbia, Vancouver, Canada
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Pierre-Yves Musso
1Department of Zoology and Life Sciences Institute, University of British Columbia, Vancouver, Canada
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Pierre Junca
1Department of Zoology and Life Sciences Institute, University of British Columbia, Vancouver, Canada
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Michael D. Gordon
1Department of Zoology and Life Sciences Institute, University of British Columbia, Vancouver, Canada
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  • For correspondence: gordon@zoology.ubc.ca
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SUMMARY

Tastes are typically thought to evoke innate appetitive or aversive behaviours, prompting food acceptance or rejection. However, research in Drosophila melanogaster indicates that taste responses can be modified through experience-dependent changes in mushroom body circuits. In this study, we develop a novel taste learning paradigm using closed-loop optogenetics. We find that appetitive and aversive taste memories can be formed by pairing gustatory stimuli with optogenetic activation of sensory or dopaminergic neurons associated with reward or punishment. As with olfactory memories, distinct dopaminergic subpopulations drive the parallel formation of short- and long-term appetitive memories. Long-term memories are protein synthesis-dependent and have energetic requirements that are satisfied by a variety of caloric food sources or by direct stimulation of MB-MP1 dopaminergic neurons. Our paradigm affords new opportunities to probe plasticity mechanisms within the taste system and understand the extent to which taste responses depend on experience.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • We have added some additional control experiments to validate the paradigm, rewritten parts of the text, and made minor updates to the graphics in the figures.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 29, 2022.
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Optogenetic induction of appetitive and aversive taste memories in Drosophila
Meghan Jelen, Pierre-Yves Musso, Pierre Junca, Michael D. Gordon
bioRxiv 2021.11.12.468444; doi: https://doi.org/10.1101/2021.11.12.468444
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Optogenetic induction of appetitive and aversive taste memories in Drosophila
Meghan Jelen, Pierre-Yves Musso, Pierre Junca, Michael D. Gordon
bioRxiv 2021.11.12.468444; doi: https://doi.org/10.1101/2021.11.12.468444

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