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Biosorption potential and molecular characterization of metal resistant autochthonous microbes from tannery solid waste

Hajira Younas, View ORCID ProfileAisha Nazir, Zakia Latif, Janice E. Thies, Muhammad Shafiq, Firdaus-e-Bareen
doi: https://doi.org/10.1101/2021.11.12.468455
Hajira Younas
1Institute of Botany, University of the Punjab, Lahore 54590, Pakistan
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Aisha Nazir
1Institute of Botany, University of the Punjab, Lahore 54590, Pakistan
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  • ORCID record for Aisha Nazir
  • For correspondence: aisha.botany@pu.edu.pk
Zakia Latif
2Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, Pakistan
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Janice E. Thies
3Department of Crop and Soil Science, Cornell University, Ithaca, NY 14853, USA
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Muhammad Shafiq
1Institute of Botany, University of the Punjab, Lahore 54590, Pakistan
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Firdaus-e-Bareen
1Institute of Botany, University of the Punjab, Lahore 54590, Pakistan
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Abstract

This study encompasses isolation and screening of heavy metal-resistant fungal and bacterial strains from tannery solid waste (TSW). Twelve fungal strains and twenty-five bacterial strains were isolated from TSW. The growth of fungal strains was observed against different heavy metals ranging from 10 mg L-1 to 1050 mg L-1 and the growth of bacteria was observed in metal concentrations ranging from 10 mg L-1 to 1200 mg L-1. Five multi-metal resistant fungal isolates belonging to the genus Trichoderma and ten bacterial isolates belonging to the genus Bacillus showed good metal resistance and biosorption potential. They were identified through molecular techniques, fungi based on ITS region ribotyping, and bacteria based on 16S rRNA ribotyping. The fungal strains were characterized as T. hamatum (TSWF-06), T. harzianum (TSWF-11), T. lixii (TSWF-02) and T. pseudokoningii (TSWF-03, TSWF-10). The bacterial strains were characterized as Bacillus xiamenensis (TSW-02), B. velezensis (TSW-05), B. piscis (TSW-06), B. safensis (TSW-10), B. subtilis (TSW-14, TSW-15, TSW-17) B. licheniformis (TSW-19), B. cereus (TSW-20) and B. thuringiensis (TSW-22). The fungal strains namely, T. pseudokoningii (TSWF-03) and T. harzianum proved to be two multi-metal resistant strains with good biosorption efficiency. Unlike fungi, bacterial strains showed metal specific resistance. The strains Bacillus xiamenensis, B. subtilis (TSW-14) and B. subtilis (TSW-15) showed good biosorption efficiency against Cr, B. safensis against Cu, B. piscis and B. subtilis (TSW-17) against Pb and B. licheniformis and B. thuringiensis against Zn. The autochthonous fungal and bacterial strains can therefore be employed to clean metal contaminated environments.

Competing Interest Statement

The authors have declared no competing interest.

  • Abbreviations

    TSW
    tannery solid waste
    HMs
    heavy metals
  • Copyright 
    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
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    Posted November 12, 2021.
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    Biosorption potential and molecular characterization of metal resistant autochthonous microbes from tannery solid waste
    Hajira Younas, Aisha Nazir, Zakia Latif, Janice E. Thies, Muhammad Shafiq, Firdaus-e-Bareen
    bioRxiv 2021.11.12.468455; doi: https://doi.org/10.1101/2021.11.12.468455
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    Biosorption potential and molecular characterization of metal resistant autochthonous microbes from tannery solid waste
    Hajira Younas, Aisha Nazir, Zakia Latif, Janice E. Thies, Muhammad Shafiq, Firdaus-e-Bareen
    bioRxiv 2021.11.12.468455; doi: https://doi.org/10.1101/2021.11.12.468455

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