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Cortical microtubule pulling forces contribute to the union of the parental genomes in the C. elegans zygote

View ORCID ProfileG. Velez-Aguilera, B. Ossareh-Nazari, L. Van Hove, N. Joly, View ORCID ProfileL. Pintard
doi: https://doi.org/10.1101/2021.11.19.469242
G. Velez-Aguilera
Programme Equipe Labélisée Ligue Contre le Cancer - Team Cell Cycle & Development - Université de Paris, CNRS, Institut Jacques Monod, F-75013, Paris, France
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  • ORCID record for G. Velez-Aguilera
B. Ossareh-Nazari
Programme Equipe Labélisée Ligue Contre le Cancer - Team Cell Cycle & Development - Université de Paris, CNRS, Institut Jacques Monod, F-75013, Paris, France
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L. Van Hove
Programme Equipe Labélisée Ligue Contre le Cancer - Team Cell Cycle & Development - Université de Paris, CNRS, Institut Jacques Monod, F-75013, Paris, France
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N. Joly
Programme Equipe Labélisée Ligue Contre le Cancer - Team Cell Cycle & Development - Université de Paris, CNRS, Institut Jacques Monod, F-75013, Paris, France
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L. Pintard
Programme Equipe Labélisée Ligue Contre le Cancer - Team Cell Cycle & Development - Université de Paris, CNRS, Institut Jacques Monod, F-75013, Paris, France
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  • For correspondence: Lionel.pintard@ijm.fr
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Abstract

Previously, we reported that the Polo-like kinase PLK-1 phosphorylates the single C. elegans lamin (LMN-1) to trigger lamina depolymerization during mitosis. We showed that this event is required for the formation of a pronuclear envelopes scission event that removes membranes on the juxtaposed oocyte and sperm pronuclear envelopes in the zygote, allowing the parental chromosomes to merge in a single nucleus after segregation (Velez-Aguilera, 2020). Here we show that cortical microtubule pulling forces contribute to pronuclear envelopes scission by promoting mitotic spindle elongation. We also demonstrate that weakening of the pronuclear envelopes, via PLK-1-mediated lamina depolymerization, is a prerequisite for the astral microtubule pulling forces to trigger pronuclear membranes scission. Finally, we provide evidence that PLK-1 mainly acts via lamina depolymerization in this process. These observations thus indicate that temporal coordination between lamina depolymerization and mitotic spindle elongation facilitates pronuclear envelopes scission and parental genomes unification.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted November 19, 2021.
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Cortical microtubule pulling forces contribute to the union of the parental genomes in the C. elegans zygote
G. Velez-Aguilera, B. Ossareh-Nazari, L. Van Hove, N. Joly, L. Pintard
bioRxiv 2021.11.19.469242; doi: https://doi.org/10.1101/2021.11.19.469242
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Cortical microtubule pulling forces contribute to the union of the parental genomes in the C. elegans zygote
G. Velez-Aguilera, B. Ossareh-Nazari, L. Van Hove, N. Joly, L. Pintard
bioRxiv 2021.11.19.469242; doi: https://doi.org/10.1101/2021.11.19.469242

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