Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Conformational Changes in the Negative Arm of the Circadian Clock Correlate with Dynamic Interactomes Involved in Post-transcriptionally Regulated Processes

View ORCID ProfileJacqueline F. Pelham, Alexander E. Mosier, Samuel C. Altshuler, Christopher L. Kirchhoff, View ORCID ProfileWilliam B. Fall, Lisa S. Baik, View ORCID ProfileJoanna C. Chiu, View ORCID ProfileJennifer M. Hurley
doi: https://doi.org/10.1101/2021.11.20.469315
Jacqueline F. Pelham
1Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jacqueline F. Pelham
Alexander E. Mosier
1Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Samuel C. Altshuler
1Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christopher L. Kirchhoff
1Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
William B. Fall
1Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for William B. Fall
Lisa S. Baik
2Department of Entomology and Nematology, University of California Davis, CA, 95616, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joanna C. Chiu
2Department of Entomology and Nematology, University of California Davis, CA, 95616, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Joanna C. Chiu
Jennifer M. Hurley
1Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States
3Center for Biotechnology and Interdisciplinary Sciences, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jennifer M. Hurley
  • For correspondence: hurlej2@rpi.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

SUMMARY

The circadian clock employs a transcriptional/translational negative feedback loop (TTFL) to anticipate environmental changes due to the Earth’s diurnal cycle, with regulation of organismal physiology believed to stem from temporal transcriptional activation by the positive arm. However, up to 80% of oscillating proteins do not have rhythmic mRNA, establishing circadian post-transcriptional regulation through unknown mechanisms. Given the pervasive conservation of the intrinsically disordered nature of negative-arm clock proteins, we hypothesized that post-transcriptional regulation may stem from conformational shifts in negative-arm proteins that time vacillations in the constituents of negative-arm macromolecular complexes to time cellular physiology. Our investigation of the negative arm clock protein in Neurospora crassa, FREQUENCY (FRQ), demonstrated temporal conformational fluidity correlated with daily changes in physiologically diverse macromolecular complex components. A parallel investigation of the macromolecular complexes centered around Drosophila melanogaster PERIOD (dPER) and human PERIOD (hPER2) found a similar number and physiological diversity of interacting partners in higher eukaryotes. Short linear motifs (SLiMs) associated with the interactors localized to disordered and phosphorylated regions on the PERs and FRQ, with disordered interactors oscillating in the macromolecular complexes over circadian time. This oscillation correlated with oscillations in post-transcriptionally regulated proteins, suggesting the negative arm may tune cellular physiology and proteostasis post-transcriptionally via vacillations in the circadian negative-arm macromolecular protein complexes.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵5 Lead Contact

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted November 20, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Conformational Changes in the Negative Arm of the Circadian Clock Correlate with Dynamic Interactomes Involved in Post-transcriptionally Regulated Processes
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Conformational Changes in the Negative Arm of the Circadian Clock Correlate with Dynamic Interactomes Involved in Post-transcriptionally Regulated Processes
Jacqueline F. Pelham, Alexander E. Mosier, Samuel C. Altshuler, Christopher L. Kirchhoff, William B. Fall, Lisa S. Baik, Joanna C. Chiu, Jennifer M. Hurley
bioRxiv 2021.11.20.469315; doi: https://doi.org/10.1101/2021.11.20.469315
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Conformational Changes in the Negative Arm of the Circadian Clock Correlate with Dynamic Interactomes Involved in Post-transcriptionally Regulated Processes
Jacqueline F. Pelham, Alexander E. Mosier, Samuel C. Altshuler, Christopher L. Kirchhoff, William B. Fall, Lisa S. Baik, Joanna C. Chiu, Jennifer M. Hurley
bioRxiv 2021.11.20.469315; doi: https://doi.org/10.1101/2021.11.20.469315

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Molecular Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4116)
  • Biochemistry (8820)
  • Bioengineering (6523)
  • Bioinformatics (23469)
  • Biophysics (11798)
  • Cancer Biology (9216)
  • Cell Biology (13327)
  • Clinical Trials (138)
  • Developmental Biology (7440)
  • Ecology (11417)
  • Epidemiology (2066)
  • Evolutionary Biology (15160)
  • Genetics (10442)
  • Genomics (14050)
  • Immunology (9176)
  • Microbiology (22170)
  • Molecular Biology (8817)
  • Neuroscience (47600)
  • Paleontology (350)
  • Pathology (1429)
  • Pharmacology and Toxicology (2492)
  • Physiology (3733)
  • Plant Biology (8084)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6039)
  • Zoology (1254)