Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Transection injury differentially alters the proteome of the human sural nerve

View ORCID ProfileMonica J. Chau, View ORCID ProfileJorge E. Quintero, Eric Blalock, Christopher Samaan, Greg Gerhardt, Craig van Horne
doi: https://doi.org/10.1101/2021.11.23.469670
Monica J. Chau
1Brain Restoration Center, College of Medicine, University of Kentucky, Lexington, KY, United States of America
2Department of Neurosurgery, College of Medicine, University of Kentucky, Lexington, KY, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Monica J. Chau
Jorge E. Quintero
1Brain Restoration Center, College of Medicine, University of Kentucky, Lexington, KY, United States of America
2Department of Neurosurgery, College of Medicine, University of Kentucky, Lexington, KY, United States of America
3Department of Neuroscience, College of Medicine, University of Kentucky, Lexington, KY, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jorge E. Quintero
Eric Blalock
4Department of Pharmacology and Nutritional Sciences, College of Medicine, University of Kentucky, Lexington, KY, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christopher Samaan
1Brain Restoration Center, College of Medicine, University of Kentucky, Lexington, KY, United States of America
2Department of Neurosurgery, College of Medicine, University of Kentucky, Lexington, KY, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Greg Gerhardt
1Brain Restoration Center, College of Medicine, University of Kentucky, Lexington, KY, United States of America
2Department of Neurosurgery, College of Medicine, University of Kentucky, Lexington, KY, United States of America
3Department of Neuroscience, College of Medicine, University of Kentucky, Lexington, KY, United States of America
5Department of Neurology, College of Medicine, University of Kentucky, Lexington, KY, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Craig van Horne
1Brain Restoration Center, College of Medicine, University of Kentucky, Lexington, KY, United States of America
2Department of Neurosurgery, College of Medicine, University of Kentucky, Lexington, KY, United States of America
3Department of Neuroscience, College of Medicine, University of Kentucky, Lexington, KY, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: craigvanhorne@uky.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Regeneration after severe peripheral nerve injury is often poor. Knowledge of human nerve regeneration and the growth microenvironment is greatly lacking. We aimed to identify the regenerative proteins in human peripheral nerve by comparing the proteome before and after a transection injury. In a unique study design, we collected from the same participants, samples from naïve and degenerating sural nerve. Naïve and degenerating (two weeks after injury) samples were analyzed using mass spectrometry and immunoassays. Using a correlation matrix, we found significantly altered levels following the nerve injury. Mass spectrometry revealed that post-injury samples had 672 proteins significantly upregulated and 661 significantly downregulated compared to naïve samples (q < 0.05, |FC| > 2). We used Gene Ontology pathways to highlight groups of proteins that were significantly upregulated or downregulated with injury-induced degeneration and regeneration. Significant protein changes in key pathways were identified including growth factor levels, Schwann cell de-differentiation, myelination downregulation, epithelial-mesenchymal transition, and axonal regeneration pathways. Having proteome signatures of human peripheral nerves of both the uninjured and the degenerating/regenerating state may serve as biomarkers to aid in the future development of repair strategies and in monitoring neural tissue regeneration.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted November 23, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Transection injury differentially alters the proteome of the human sural nerve
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Transection injury differentially alters the proteome of the human sural nerve
Monica J. Chau, Jorge E. Quintero, Eric Blalock, Christopher Samaan, Greg Gerhardt, Craig van Horne
bioRxiv 2021.11.23.469670; doi: https://doi.org/10.1101/2021.11.23.469670
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Transection injury differentially alters the proteome of the human sural nerve
Monica J. Chau, Jorge E. Quintero, Eric Blalock, Christopher Samaan, Greg Gerhardt, Craig van Horne
bioRxiv 2021.11.23.469670; doi: https://doi.org/10.1101/2021.11.23.469670

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3514)
  • Biochemistry (7364)
  • Bioengineering (5341)
  • Bioinformatics (20316)
  • Biophysics (10038)
  • Cancer Biology (7769)
  • Cell Biology (11342)
  • Clinical Trials (138)
  • Developmental Biology (6445)
  • Ecology (9977)
  • Epidemiology (2065)
  • Evolutionary Biology (13351)
  • Genetics (9369)
  • Genomics (12603)
  • Immunology (7724)
  • Microbiology (19083)
  • Molecular Biology (7458)
  • Neuroscience (41125)
  • Paleontology (300)
  • Pathology (1235)
  • Pharmacology and Toxicology (2142)
  • Physiology (3174)
  • Plant Biology (6873)
  • Scientific Communication and Education (1276)
  • Synthetic Biology (1900)
  • Systems Biology (5324)
  • Zoology (1091)