Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

SELENOT deficiency alters projection neuron migration during corticogenesis in mice

Emmanuelle Carpentier, Anthony Falluel-Morel, Lisa Brunet, Magalie Bénard, David Godefroy, Loubna Boukhzar, Arnaud Arabo, View ORCID ProfileYoussef Anouar
doi: https://doi.org/10.1101/2021.11.23.469805
Emmanuelle Carpentier
1University of Rouen Normandie, INSERM U1239, Neuronal and Neuroendocrine Differenciation and Communication laboratory, IRIB, CURIB, 76821 Mont-Saint-Aignan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anthony Falluel-Morel
1University of Rouen Normandie, INSERM U1239, Neuronal and Neuroendocrine Differenciation and Communication laboratory, IRIB, CURIB, 76821 Mont-Saint-Aignan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lisa Brunet
1University of Rouen Normandie, INSERM U1239, Neuronal and Neuroendocrine Differenciation and Communication laboratory, IRIB, CURIB, 76821 Mont-Saint-Aignan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Magalie Bénard
2University of Rouen Normandie, INSERM, the Cell Imaging Platform of Normandy PRIMACEN, IRIB, CURIB, 76821 Mont-Saint-Aignan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David Godefroy
1University of Rouen Normandie, INSERM U1239, Neuronal and Neuroendocrine Differenciation and Communication laboratory, IRIB, CURIB, 76821 Mont-Saint-Aignan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Loubna Boukhzar
1University of Rouen Normandie, INSERM U1239, Neuronal and Neuroendocrine Differenciation and Communication laboratory, IRIB, CURIB, 76821 Mont-Saint-Aignan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Arnaud Arabo
3University of Rouen Normandie, Biological Resource Facility, CURIB, 76821 Mont-Saint-Aignan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Youssef Anouar
1University of Rouen Normandie, INSERM U1239, Neuronal and Neuroendocrine Differenciation and Communication laboratory, IRIB, CURIB, 76821 Mont-Saint-Aignan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Youssef Anouar
  • For correspondence: youssef.anouar@univ-rouen.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

During corticogenesis, projection neurons migrate along the radial glial axis to form cortical layers, the alteration of which is associated with functional deficits in adulthood. As byproducts of cell metabolism, reactive oxygen species act as second messengers to contribute to neurodevelopment; however, free radical excess may impede this process. SELENOT is a thioredoxin-like enzyme of the endoplasmic reticulum abundantly expressed during embryogenesis whose gene disruption in the brain leads to neuroblast cell demise due to increased free radical levels. To determine the potential contribution of SELENOT to the establishment of cortical networks, we analyzed first its expression profile in the neocortex at different stages of development. These studies revealed the widespread expression of SELENOT in all cortical layers, and its continous increase throughout mouse lifespan. In addition, we disrupted the SELENOT gene in the cortex using in utero electroporation and Nes-Cre/lox knockout. SELENOT deficiency altered neuroblast migration polarity, at the level of radial scaffolding, and projection neuron positionning. These results indicate that SELENOT plays a crucial role during neurodevelopment by sustaining projection neuron migration.

Competing Interest Statement

The authors have declared no competing interest.

  • Abbreviations

    CP
    cortical plate
    En
    embryonic day n
    GE
    ganglionic eminence
    IUE
    in utero electroporation
    IZ
    intermediate zone
    PND
    postnatal day
    ROS
    reactive oxygen species
    SELENOT
    selenoprotein T
    SP
    subplate
    SVZ
    subventricular zone
    VZ
    ventricular zone
  • Copyright 
    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
    Back to top
    PreviousNext
    Posted November 24, 2021.
    Download PDF
    Email

    Thank you for your interest in spreading the word about bioRxiv.

    NOTE: Your email address is requested solely to identify you as the sender of this article.

    Enter multiple addresses on separate lines or separate them with commas.
    SELENOT deficiency alters projection neuron migration during corticogenesis in mice
    (Your Name) has forwarded a page to you from bioRxiv
    (Your Name) thought you would like to see this page from the bioRxiv website.
    CAPTCHA
    This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
    Share
    SELENOT deficiency alters projection neuron migration during corticogenesis in mice
    Emmanuelle Carpentier, Anthony Falluel-Morel, Lisa Brunet, Magalie Bénard, David Godefroy, Loubna Boukhzar, Arnaud Arabo, Youssef Anouar
    bioRxiv 2021.11.23.469805; doi: https://doi.org/10.1101/2021.11.23.469805
    Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
    Citation Tools
    SELENOT deficiency alters projection neuron migration during corticogenesis in mice
    Emmanuelle Carpentier, Anthony Falluel-Morel, Lisa Brunet, Magalie Bénard, David Godefroy, Loubna Boukhzar, Arnaud Arabo, Youssef Anouar
    bioRxiv 2021.11.23.469805; doi: https://doi.org/10.1101/2021.11.23.469805

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    • Tweet Widget
    • Facebook Like
    • Google Plus One

    Subject Area

    • Developmental Biology
    Subject Areas
    All Articles
    • Animal Behavior and Cognition (3484)
    • Biochemistry (7336)
    • Bioengineering (5308)
    • Bioinformatics (20224)
    • Biophysics (9991)
    • Cancer Biology (7716)
    • Cell Biology (11280)
    • Clinical Trials (138)
    • Developmental Biology (6426)
    • Ecology (9930)
    • Epidemiology (2065)
    • Evolutionary Biology (13297)
    • Genetics (9354)
    • Genomics (12566)
    • Immunology (7686)
    • Microbiology (18979)
    • Molecular Biology (7428)
    • Neuroscience (40944)
    • Paleontology (300)
    • Pathology (1226)
    • Pharmacology and Toxicology (2132)
    • Physiology (3146)
    • Plant Biology (6850)
    • Scientific Communication and Education (1272)
    • Synthetic Biology (1893)
    • Systems Biology (5306)
    • Zoology (1087)