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Stable and functionally diverse versatile peroxidases by computational design directly from sequence

Shiran Barber-Zucker, Vladimir Mindel, Eva Garcia-Ruiz, Jonathan J. Weinstein, Miguel Alcalde, Sarel J. Fleishman
doi: https://doi.org/10.1101/2021.11.25.469886
Shiran Barber-Zucker
1Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7600001, Israel
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Vladimir Mindel
1Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7600001, Israel
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Eva Garcia-Ruiz
2Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, Madrid 28094, Spain
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Jonathan J. Weinstein
1Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7600001, Israel
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Miguel Alcalde
2Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, Madrid 28094, Spain
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Sarel J. Fleishman
1Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7600001, Israel
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  • For correspondence: sarel.fleishman@weizmann.ac.il
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ABSTRACT

White-rot fungi secrete a repertoire of high-redox potential oxidoreductases to efficiently decompose lignin. Of these enzymes, versatile peroxidases (VPs) are the most promiscuous biocatalysts. VPs are attractive enzymes for research and industrial use, but their recombinant production is extremely challenging. To date, only a single VP has been structurally characterized and optimized for recombinant functional expression, stability and activity. Computational enzyme optimization methods can be applied to many enzymes in parallel, but they require accurate structures. Here, we demonstrate that model structures computed by deep-learning based ab initio structure prediction methods are reliable starting points for one-shot PROSS stability-design calculations. Four designed VPs encoding as many as 43 mutations relative to the wild type enzymes are functionally expressed in yeast whereas their wild type parents are not. Three of these designs exhibit substantial and useful diversity in reactivity profile and tolerance to environmental conditions. The reliability of the new generation of structure predictors and design methods increases the scale and scope of computational enzyme optimization, enabling efficient discovery and exploitation of the functional diversity in natural enzyme families.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵* sarel{at}weizmann.ac.il

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 25, 2021.
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Stable and functionally diverse versatile peroxidases by computational design directly from sequence
Shiran Barber-Zucker, Vladimir Mindel, Eva Garcia-Ruiz, Jonathan J. Weinstein, Miguel Alcalde, Sarel J. Fleishman
bioRxiv 2021.11.25.469886; doi: https://doi.org/10.1101/2021.11.25.469886
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Stable and functionally diverse versatile peroxidases by computational design directly from sequence
Shiran Barber-Zucker, Vladimir Mindel, Eva Garcia-Ruiz, Jonathan J. Weinstein, Miguel Alcalde, Sarel J. Fleishman
bioRxiv 2021.11.25.469886; doi: https://doi.org/10.1101/2021.11.25.469886

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