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Structural and functional organization of visual responses in the inferior olive of larval zebrafish

View ORCID ProfileRita Felix, View ORCID ProfileDaniil A Markov, View ORCID ProfileSabine L. Renninger, View ORCID ProfileRaquel Tomás, View ORCID ProfileAlexandre Laborde, View ORCID ProfileMegan R Carey, View ORCID ProfileMichael B Orger, View ORCID ProfileRuben Portugues
doi: https://doi.org/10.1101/2021.11.29.470378
Rita Felix
1Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal 1400-038
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Daniil A Markov
2Max Planck Institute of Neurobiology, Sensorimotor Control Research Group, Martinsried, Germany
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Sabine L. Renninger
1Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal 1400-038
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  • ORCID record for Sabine L. Renninger
Raquel Tomás
1Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal 1400-038
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Alexandre Laborde
1Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal 1400-038
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Megan R Carey
1Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal 1400-038
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Michael B Orger
1Champalimaud Research, Champalimaud Centre for the Unknown, Lisbon, Portugal 1400-038
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  • For correspondence: michael.orger@neuro.fchampalimaud.org ruben.portugues@tum.de
Ruben Portugues
2Max Planck Institute of Neurobiology, Sensorimotor Control Research Group, Martinsried, Germany
3Institute of Neuroscience, Technical University of Munich, Biedersteiner Strasse 29, 80802 Munich, Germany
4Munich Cluster of Systems Neurology (SyNergy), Feodor-Lynen-Strasse 17, 81377 Munich, Germany
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  • For correspondence: michael.orger@neuro.fchampalimaud.org ruben.portugues@tum.de
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Abstract

The olivo-cerebellar system plays an important role in vertebrate sensorimotor control. According to a classical theory of cerebellar cortex, the inferior olive (IO) provides Purkinje cells with error information which drives motor learning in the cerebellum. Here we investigate the sensory representations in the IO of larval zebrafish and their spatial organization. Using single-cell labeling of genetically identified IO neurons we find that they can be divided into at least two distinct groups based on their spatial location, dendritic morphology, and axonal projection patterns. In the same genetically targeted population, we recorded calcium activity in response to a set of visual stimuli using 2-photon imaging. We found that most IO neurons showed direction selective and binocular responses to visual stimuli and that functional properties were spatially organized within the IO. Light-sheet functional imaging that allowed for simultaneous activity recordings at the soma and axonal level revealed tight coupling between soma location, axonal projections and functional properties of IO neurons. Taken together, our results suggest that anatomically-defined classes of inferior olive neurons correspond to distinct functional types, and that topographic connections between IO and cerebellum contribute to organization of the cerebellum into distinct functional zones.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 30, 2021.
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Structural and functional organization of visual responses in the inferior olive of larval zebrafish
Rita Felix, Daniil A Markov, Sabine L. Renninger, Raquel Tomás, Alexandre Laborde, Megan R Carey, Michael B Orger, Ruben Portugues
bioRxiv 2021.11.29.470378; doi: https://doi.org/10.1101/2021.11.29.470378
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Structural and functional organization of visual responses in the inferior olive of larval zebrafish
Rita Felix, Daniil A Markov, Sabine L. Renninger, Raquel Tomás, Alexandre Laborde, Megan R Carey, Michael B Orger, Ruben Portugues
bioRxiv 2021.11.29.470378; doi: https://doi.org/10.1101/2021.11.29.470378

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