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RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells

Peiguo Shi, Michael R. Murphy, Alexis O. Aparicio, Jordan S. Kesner, Zhou Fang, Ziheng Chen, Aditi Trehan, View ORCID ProfileXuebing Wu
doi: https://doi.org/10.1101/2021.11.30.470032
Peiguo Shi
1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA
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  • For correspondence: ps3008@cumc.columbia.edu xw2629@cumc.columbia.edu
Michael R. Murphy
1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA
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Alexis O. Aparicio
1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA
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Jordan S. Kesner
1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA
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Zhou Fang
1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA
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Ziheng Chen
1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA
2Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
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Aditi Trehan
1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA
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Xuebing Wu
1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA
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  • ORCID record for Xuebing Wu
  • For correspondence: ps3008@cumc.columbia.edu xw2629@cumc.columbia.edu
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ABSTRACT

While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted November 30, 2021.
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RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells
Peiguo Shi, Michael R. Murphy, Alexis O. Aparicio, Jordan S. Kesner, Zhou Fang, Ziheng Chen, Aditi Trehan, Xuebing Wu
bioRxiv 2021.11.30.470032; doi: https://doi.org/10.1101/2021.11.30.470032
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RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells
Peiguo Shi, Michael R. Murphy, Alexis O. Aparicio, Jordan S. Kesner, Zhou Fang, Ziheng Chen, Aditi Trehan, Xuebing Wu
bioRxiv 2021.11.30.470032; doi: https://doi.org/10.1101/2021.11.30.470032

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