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Maternal parity affects Day 8 embryo gene expression in old mares

View ORCID ProfileEmilie Derisoud, View ORCID ProfileLuc Jouneau, Clothilde Gourtay, Anne Margat, Catherine Archilla, View ORCID ProfileYan Jaszczyszyn, View ORCID ProfileRachel Legendre, View ORCID ProfileNathalie Daniel, Nathalie Peynot, Michèle Dahirel, Laurie Briot, Frédéric De Geoffroy, View ORCID ProfileVéronique Duranthon, View ORCID ProfilePascale Chavatte-Palmer
doi: https://doi.org/10.1101/2021.12.01.470709
Emilie Derisoud
1Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France
2Ecole Nationale Vétérinaire d’Alfort, BREED, Maisons-Alfort, France
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  • ORCID record for Emilie Derisoud
  • For correspondence: emilie.derisoud@gmail.com pascale.chavatte-palmer@inrae.fr
Luc Jouneau
1Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France
2Ecole Nationale Vétérinaire d’Alfort, BREED, Maisons-Alfort, France
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Clothilde Gourtay
3IFCE, Plateau technique du Pin, Exmes, France
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Anne Margat
3IFCE, Plateau technique du Pin, Exmes, France
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Catherine Archilla
1Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France
2Ecole Nationale Vétérinaire d’Alfort, BREED, Maisons-Alfort, France
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Yan Jaszczyszyn
4Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CNRS, CEA, Paris-Sud University, Gif-sur-Yvette, France
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Rachel Legendre
5Institut Pasteur—Bioinformatics and Biostatistics Hub—Department of Computational Biology, USR 3756 IP CNRS, Paris, France
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Nathalie Daniel
1Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France
2Ecole Nationale Vétérinaire d’Alfort, BREED, Maisons-Alfort, France
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Nathalie Peynot
1Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France
2Ecole Nationale Vétérinaire d’Alfort, BREED, Maisons-Alfort, France
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Michèle Dahirel
1Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France
2Ecole Nationale Vétérinaire d’Alfort, BREED, Maisons-Alfort, France
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Laurie Briot
3IFCE, Plateau technique du Pin, Exmes, France
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Frédéric De Geoffroy
3IFCE, Plateau technique du Pin, Exmes, France
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Véronique Duranthon
1Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France
2Ecole Nationale Vétérinaire d’Alfort, BREED, Maisons-Alfort, France
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Pascale Chavatte-Palmer
1Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France
2Ecole Nationale Vétérinaire d’Alfort, BREED, Maisons-Alfort, France
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  • For correspondence: emilie.derisoud@gmail.com pascale.chavatte-palmer@inrae.fr
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Abstract

As sport career is a priority in most of equine breeds, mares are frequently bred for the first time at an advanced age. Both age and first gestation were shown to have a deleterious effect on reproduction outcomes, respectively on fertility and offspring weight but the effect mare’s parity in older mares on embryo quality has never been considered. The aim of this project was to determine the effect of old mare’s nulliparity on gene expression in embryos. Day 8 post ovulation embryos were collected from old (10-16 years old) nulliparous (ON, N=5) or multiparous (OM, N=6) non-nursing Saddlebred mares, inseminated with the semen of one stallion. Pure (TE_part) or inner cell mass enriched (ICMandTE) trophoblast were obtained by embryo bisection and paired end, non-oriented RNA sequencing (Illumina, NextSeq500) was performed on each hemi-embryo. To discriminate gene expression in the ICM from that in the TE, deconvolution (DeMixT R package) was used on the ICMandTE dataset. Differential expression was analyzed (DESeq2) with embryo sex and diameter as cofactors using a false discovery rate <0.05 cutoff. Although the expression of only a few genes was altered by mare’s nulliparity (33 in ICM and 23 in TE), those genes were related to nutrient exchanges and responses to environment signaling, both in ICM and TE, suggesting that the developing environment from these mares are not optimal for embryo growth. In conclusion, being nulliparous and old does not seem to be the perfect match for embryonic development in mares.

Summary sentence Mare’s parity in old mares impacts the expression of genes related to development and molecule exchanges in ICM and TE of blastocysts suggesting an adaptation to an altered environment.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Grant support: This work was supported by the “Institut Français du Cheval et de l’Equitation” (grant numbers CS_2018_23, 2018). The National Research Institute for Agriculture, Food and Environment (INRAE) department Animal Physiology and Breeding Systems also supported this research.

  • https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE188866

  • List of abbreviations
    DEG
    differential expressed genes
    DeMixT_ICM_cells
    deconvoluted gene expression in ICM cells
    DeMixT_TE_cells
    deconvoluted gene expression in TE cells
    ECM
    Extracellular matrix
    ERR
    embryo collection rate
    FDR
    false discovery rate
    GO BP
    Gene Ontology biological process
    GO
    Gene Ontology
    GSEA
    gene set enrichment analyses ICM: inner cell mass
    ICMandTE
    inner cell mass enriched hemi-embryo ICSI: intracytoplasmic sperm injection
    KEGG
    Kyoto Encyclopedia of Genes and Genomes Log2FC: log2 fold change
    NES
    normalized enrichment score
    OM
    old multiparous mares
    ON
    old nulliparous mares
    TE
    trophoblast
    TE_part
    pure trophoblast hemi-embryo
    XIST
    X inactive Specific Transcript
  • Copyright 
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    Posted December 02, 2021.
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    Maternal parity affects Day 8 embryo gene expression in old mares
    Emilie Derisoud, Luc Jouneau, Clothilde Gourtay, Anne Margat, Catherine Archilla, Yan Jaszczyszyn, Rachel Legendre, Nathalie Daniel, Nathalie Peynot, Michèle Dahirel, Laurie Briot, Frédéric De Geoffroy, Véronique Duranthon, Pascale Chavatte-Palmer
    bioRxiv 2021.12.01.470709; doi: https://doi.org/10.1101/2021.12.01.470709
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    Maternal parity affects Day 8 embryo gene expression in old mares
    Emilie Derisoud, Luc Jouneau, Clothilde Gourtay, Anne Margat, Catherine Archilla, Yan Jaszczyszyn, Rachel Legendre, Nathalie Daniel, Nathalie Peynot, Michèle Dahirel, Laurie Briot, Frédéric De Geoffroy, Véronique Duranthon, Pascale Chavatte-Palmer
    bioRxiv 2021.12.01.470709; doi: https://doi.org/10.1101/2021.12.01.470709

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