Abstract
Adaptive sampling enables selection of individual DNA molecules from sequencing libraries, a unique property of nanopore sequencing. Here we develop our adaptive sampling tool readfish to become “barcode-aware” enabling selection of different targets within barcoded samples or filtering out individual barcodes. We show that multiple human genomes can be assessed for copy number and structural variation on a single sequencing flow cell using sample specific customised target panels on both GridION and PromethION devices.
Competing Interest Statement
ML was a member of the MinION access program and has received free flow cells and sequencing reagents in the past. ML has received reimbursement for travel, accommodation and conference fees to speak at events organised by Oxford Nanopore Technologies. Early access to MinKNOW software updates from Oxford Nanopore Technologies enabled this work.
Footnotes
This text has been revised to incorporate new data generated on the Oxford Nanopore PromethION platform.
