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Retracing the evolution from antimicrobial to targeting peptides

View ORCID ProfileO.D. Caspari, View ORCID ProfileC. Garrido, View ORCID ProfileY. Choquet, View ORCID ProfileF.-A. Wollman, View ORCID ProfileI Lafontaine
doi: https://doi.org/10.1101/2021.12.03.471120
O.D. Caspari
1Institut de Biologie Physico-Chimique (CNRS/Sorbonne Université), 13 Rue Pierre et Marie Curie, 75005 Paris, France
2Department of Microbiology, Institut Pasteur, 28 Rue Docteur Roux, 75015 Paris, France
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  • For correspondence: odc20@cantab.net ingrid.lafontaine@ibpc.fr
C. Garrido
1Institut de Biologie Physico-Chimique (CNRS/Sorbonne Université), 13 Rue Pierre et Marie Curie, 75005 Paris, France
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Y. Choquet
1Institut de Biologie Physico-Chimique (CNRS/Sorbonne Université), 13 Rue Pierre et Marie Curie, 75005 Paris, France
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F.-A. Wollman
1Institut de Biologie Physico-Chimique (CNRS/Sorbonne Université), 13 Rue Pierre et Marie Curie, 75005 Paris, France
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I Lafontaine
1Institut de Biologie Physico-Chimique (CNRS/Sorbonne Université), 13 Rue Pierre et Marie Curie, 75005 Paris, France
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  • For correspondence: odc20@cantab.net ingrid.lafontaine@ibpc.fr
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Abstract

We experimentally challenged the endosymbiotic hypothesis that organelle-targeting peptides derive from antimicrobial amphipathic peptides delivered by the host cell, to which organelle progenitors became resistant. To explore the molecular changes required to convert such antimicrobial peptides into bona fide organelle-targeting peptides, we expressed a set of 13 antimicrobial peptides of various origins in the green alga Chlamydomonas reinhardtii that serves as a model for both mitochondrial and chloroplast import. The peptides were modified to match distinctive features of mitochondrial and chloroplast targeting peptides, and we assessed their targeting potential by following the intracellular localization and maturation of a Venus fluorescent reporter used as cargo protein. We present a temporal evolutionary scenario that emphasizes the early contribution of exchanging Lysines with Arginines in the sequence of the antimicrobial peptide, the evolution of a processing site followed by the addition of unstructured sequence and protein interaction sites that allow the selective targeting to the chloroplast.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 03, 2021.
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Retracing the evolution from antimicrobial to targeting peptides
O.D. Caspari, C. Garrido, Y. Choquet, F.-A. Wollman, I Lafontaine
bioRxiv 2021.12.03.471120; doi: https://doi.org/10.1101/2021.12.03.471120
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Retracing the evolution from antimicrobial to targeting peptides
O.D. Caspari, C. Garrido, Y. Choquet, F.-A. Wollman, I Lafontaine
bioRxiv 2021.12.03.471120; doi: https://doi.org/10.1101/2021.12.03.471120

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