Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Mechanical stretch regulates macropinocytosis in Hydra vulgaris

View ORCID ProfileTaylor D. Skokan, View ORCID ProfileBert Hobmayer, View ORCID ProfileKara L. McKinley, View ORCID ProfileRonald D. Vale
doi: https://doi.org/10.1101/2021.12.03.471193
Taylor D. Skokan
1Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Taylor D. Skokan
Bert Hobmayer
2Department for Evolutionary Developmental Biology, Institute of Zoology and Centre for Molecular Biosciences, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bert Hobmayer
Kara L. McKinley
3Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kara L. McKinley
  • For correspondence: valer@janelia.hhmi.org kara_mckinley@harvard.edu
Ronald D. Vale
1Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
4Howard Hughes Medical Institute Janelia Research Campus, Ashburn, VA, 20147, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ronald D. Vale
  • For correspondence: valer@janelia.hhmi.org kara_mckinley@harvard.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Cells rely on a diverse array of engulfment processes to sense, exploit, and adapt to their environments. Macropinocytosis is a versatile example of such a process, allowing for the indiscriminate and rapid uptake of large volumes of fluid and membrane. Much of the molecular machinery essential for macropinocytosis has been well established. However, most of these studies relied on tissue culture models, leaving the regulation of this process within the context of organs and organisms unresolved. Here, we report that large-scale macropinocytosis occurs in the outer epithelial layer of the cnidarian Hydra vulgaris. Exploiting Hydra’s relatively simple body plan, we developed approaches to visualize macropinocytosis over extended periods of time in living tissue, revealing constitutive engulfment across the entire body axis. Using pharmacological perturbations, we establish a role for stretch-activated channels, including Piezo, and downstream calcium influx in inhibiting this process. Finally, we show that the direct application of planar stretch leads to calcium influx and a corresponding inhibition of macropinocytosis. Together, our approaches provide a platform for the mechanistic dissection of constitutive macropinocytosis in physiological contexts and reveal a role for macropinocytosis in responding to membrane tension.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted December 04, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Mechanical stretch regulates macropinocytosis in Hydra vulgaris
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Mechanical stretch regulates macropinocytosis in Hydra vulgaris
Taylor D. Skokan, Bert Hobmayer, Kara L. McKinley, Ronald D. Vale
bioRxiv 2021.12.03.471193; doi: https://doi.org/10.1101/2021.12.03.471193
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Mechanical stretch regulates macropinocytosis in Hydra vulgaris
Taylor D. Skokan, Bert Hobmayer, Kara L. McKinley, Ronald D. Vale
bioRxiv 2021.12.03.471193; doi: https://doi.org/10.1101/2021.12.03.471193

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3484)
  • Biochemistry (7336)
  • Bioengineering (5308)
  • Bioinformatics (20225)
  • Biophysics (9991)
  • Cancer Biology (7717)
  • Cell Biology (11280)
  • Clinical Trials (138)
  • Developmental Biology (6426)
  • Ecology (9930)
  • Epidemiology (2065)
  • Evolutionary Biology (13298)
  • Genetics (9354)
  • Genomics (12566)
  • Immunology (7687)
  • Microbiology (18979)
  • Molecular Biology (7428)
  • Neuroscience (40944)
  • Paleontology (300)
  • Pathology (1226)
  • Pharmacology and Toxicology (2132)
  • Physiology (3146)
  • Plant Biology (6850)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1893)
  • Systems Biology (5306)
  • Zoology (1087)