Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines

View ORCID ProfileStephanie N. Seifert, Shuangyi Bai, Stephen Fawcett, Elizabeth B. Norton, Kevin J. Zwezdaryk, James Robinson, Bronwyn Gunn, View ORCID ProfileMichael C. Letko
doi: https://doi.org/10.1101/2021.12.05.471310
Stephanie N. Seifert
1Paul G. Allen School for Global Health, Washington State University, Pullman, WA, U.S.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stephanie N. Seifert
Shuangyi Bai
1Paul G. Allen School for Global Health, Washington State University, Pullman, WA, U.S.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephen Fawcett
1Paul G. Allen School for Global Health, Washington State University, Pullman, WA, U.S.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth B. Norton
3Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kevin J. Zwezdaryk
3Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
James Robinson
2Department of Pediatrics, Tulane University School of Medicine, New Orleans, LA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bronwyn Gunn
1Paul G. Allen School for Global Health, Washington State University, Pullman, WA, U.S.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael C. Letko
1Paul G. Allen School for Global Health, Washington State University, Pullman, WA, U.S.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michael C. Letko
  • For correspondence: michael.letko@wsu.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Spillover of sarbecoviruses from animals to humans has resulted in outbreaks of severe acute respiratory syndrome SARS-CoVs and the ongoing COVID-19 pandemic. Efforts to identify the origins of SARS-CoV-1 and −2 has resulted in the discovery of numerous animal sarbecoviruses – the majority of which are only distantly related to known human pathogens and do not infect human cells. The receptor binding domain (RBD) on sarbecoviruses engages receptor molecules on the host cell and mediates cell invasion. Here, we tested the receptor tropism and serological cross reactivity for RBDs from two sarbecoviruses found in Russian horseshoe bats. While these two viruses are in a viral lineage distinct from SARS-CoV-1 and −2, one virus, Khosta-2, was capable of using human ACE2 to facilitate cell entry. Viral pseudotypes with a recombinant, SARS-CoV-2 spike encoding for the Khosta 2 RBD were resistant to both SARS-CoV-2 monoclonal antibodies and serum from individuals vaccinated for SARS-CoV-2. Our findings further demonstrate that sarbecoviruses circulating in wildlife outside of Asia also pose a threat to global health and ongoing vaccine campaigns against SARS-CoV-2

ONE SENTENCE SUMMARY European bat coronaviruses that are only distantly related to SARS-CoV-2 but use the same cell entry route, escape the immune response against SARS-CoV-2 vaccines, driving the need for broader vaccines.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • We have now added more experimental data to the manuscript. In figure 2 we tested the cell tropism of full-length Khosta spikes. In figure 3 we tested neutralization efficacy of SARS-CoV-2 monoclonal antibodies and serum from SARS-CoV-2 vaccinated individuals against a chimeric spike protein carrying part of the Khosta spike.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted April 01, 2022.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
Stephanie N. Seifert, Shuangyi Bai, Stephen Fawcett, Elizabeth B. Norton, Kevin J. Zwezdaryk, James Robinson, Bronwyn Gunn, Michael C. Letko
bioRxiv 2021.12.05.471310; doi: https://doi.org/10.1101/2021.12.05.471310
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
An ACE2-dependent Sarbecovirus in Russian bats is resistant to SARS-CoV-2 vaccines
Stephanie N. Seifert, Shuangyi Bai, Stephen Fawcett, Elizabeth B. Norton, Kevin J. Zwezdaryk, James Robinson, Bronwyn Gunn, Michael C. Letko
bioRxiv 2021.12.05.471310; doi: https://doi.org/10.1101/2021.12.05.471310

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4113)
  • Biochemistry (8816)
  • Bioengineering (6519)
  • Bioinformatics (23463)
  • Biophysics (11791)
  • Cancer Biology (9209)
  • Cell Biology (13324)
  • Clinical Trials (138)
  • Developmental Biology (7439)
  • Ecology (11410)
  • Epidemiology (2066)
  • Evolutionary Biology (15152)
  • Genetics (10439)
  • Genomics (14044)
  • Immunology (9171)
  • Microbiology (22155)
  • Molecular Biology (8812)
  • Neuroscience (47571)
  • Paleontology (350)
  • Pathology (1428)
  • Pharmacology and Toxicology (2491)
  • Physiology (3730)
  • Plant Biology (8081)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6038)
  • Zoology (1253)