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Patterned mechanical feedback establishes a global myosin gradient

Hannah J. Gustafson, View ORCID ProfileNikolas Claussen, View ORCID ProfileStefano De Renzis, View ORCID ProfileSebastian J. Streichan
doi: https://doi.org/10.1101/2021.12.06.471321
Hannah J. Gustafson
1Department of Physics, University of California Santa Barbara, Santa Barbara, California 93106, USA
2Biomolecular Science and Engineering, University of California Santa Barbara, Santa Barbara, California 93106, USA
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Nikolas Claussen
1Department of Physics, University of California Santa Barbara, Santa Barbara, California 93106, USA
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Stefano De Renzis
3EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
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Sebastian J. Streichan
1Department of Physics, University of California Santa Barbara, Santa Barbara, California 93106, USA
2Biomolecular Science and Engineering, University of California Santa Barbara, Santa Barbara, California 93106, USA
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  • For correspondence: streicha@ucsb.edu
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Abstract

Morphogenesis, the coordinated execution of developmental programs that shape embryos, raises many fundamental questions at the interface between physics and biology. In particular, how the dynamics of active cytoskeletal processes are coordinated across the surface of entire embryos to generate global cell flows is poorly understood. Two distinct regulatory principles have been identified: genetic programs and dynamic response to mechanical stimuli. Despite progress, disentangling these two contributions remains challenging. Here, we combine in toto light sheet microscopy with genetic and optogenetic perturbations of tissue mechanics to examine theoretically predicted dynamic recruitment of non-muscle myosin II to cell junctions during Drosophila embryogenesis. We find dynamic recruitment has a long-range impact on global myosin configuration, and the rate of junction deformation sets the rate of myosin recruitment. Mathematical modeling and high frequency analysis reveal myosin fluctuations on junctions around a mean value set by mechanical feedback. Our model accounts for the early establishment of the global myosin pattern at 80% fidelity. Taken together our results indicate spatially modulated mechanical feedback as a key regulatory input in the establishment of long-range gradients of cytoskeletal configurations and global tissue flow patterns.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 07, 2021.
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Patterned mechanical feedback establishes a global myosin gradient
Hannah J. Gustafson, Nikolas Claussen, Stefano De Renzis, Sebastian J. Streichan
bioRxiv 2021.12.06.471321; doi: https://doi.org/10.1101/2021.12.06.471321
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Patterned mechanical feedback establishes a global myosin gradient
Hannah J. Gustafson, Nikolas Claussen, Stefano De Renzis, Sebastian J. Streichan
bioRxiv 2021.12.06.471321; doi: https://doi.org/10.1101/2021.12.06.471321

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