Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The SUMO protease Ulp2 regulates genome stability and drug resistance in the human fungal pathogen Candida albicans

View ORCID ProfileMarzia Rizzo, Natthapon Soisangwan, Jan Soetaert, Samuel Vega-Estevez, View ORCID ProfileAnna Selmecki, View ORCID ProfileAlessia Buscaino
doi: https://doi.org/10.1101/2021.12.06.471441
Marzia Rizzo
1University of Kent, School of Biosciences, Kent Fungal Group, Canterbury Kent, CT2 7NJ, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marzia Rizzo
Natthapon Soisangwan
2University of Minnesota, Department of Microbiology and Immunology, Minneapolis, Minnesota, United States, 55455
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jan Soetaert
3Blizard Advanced Light Microscopy (BALM), Queen Mary University of London, London E12AT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Samuel Vega-Estevez
1University of Kent, School of Biosciences, Kent Fungal Group, Canterbury Kent, CT2 7NJ, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anna Selmecki
2University of Minnesota, Department of Microbiology and Immunology, Minneapolis, Minnesota, United States, 55455
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Anna Selmecki
Alessia Buscaino
1University of Kent, School of Biosciences, Kent Fungal Group, Canterbury Kent, CT2 7NJ, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alessia Buscaino
  • For correspondence: A.Buscaino@kent.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Stress-induced genome instability in microbial organisms is emerging as a critical regulatory mechanism for driving rapid and reversible adaption to drastic environmental changes. In Candida albicans, a human fungal pathogen that causes life-threatening infections, genome plasticity confers increased virulence and antifungal drug resistance. Discovering the mechanisms regulating C. albicans genome plasticity is a priority to understand how this and other microbial pathogens establish life-threatening infections and develop resistance to antifungal drugs. We identified the SUMO protease Ulp2 as a critical regulator of C. albicans genome integrity through genetic screening. Deletion of ULP2 leads to hypersensitivity to genotoxic agents and increased genome instability. This increased genome diversity causes reduced fitness under standard laboratory growth conditions but enhances adaptation to stress, making ulp2Δ/Δ cells more likely to thrive in the presence of antifungal drugs. Whole-genome sequencing indicates that ulp2Δ/Δ cells counteract antifungal drug-induced stress by developing segmental aneuploidies of chromosome R and chromosome I. We demonstrate that intrachromosomal repetitive elements drive the formation of complex novel genotypes with adaptive power.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted December 07, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The SUMO protease Ulp2 regulates genome stability and drug resistance in the human fungal pathogen Candida albicans
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The SUMO protease Ulp2 regulates genome stability and drug resistance in the human fungal pathogen Candida albicans
Marzia Rizzo, Natthapon Soisangwan, Jan Soetaert, Samuel Vega-Estevez, Anna Selmecki, Alessia Buscaino
bioRxiv 2021.12.06.471441; doi: https://doi.org/10.1101/2021.12.06.471441
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
The SUMO protease Ulp2 regulates genome stability and drug resistance in the human fungal pathogen Candida albicans
Marzia Rizzo, Natthapon Soisangwan, Jan Soetaert, Samuel Vega-Estevez, Anna Selmecki, Alessia Buscaino
bioRxiv 2021.12.06.471441; doi: https://doi.org/10.1101/2021.12.06.471441

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3484)
  • Biochemistry (7336)
  • Bioengineering (5308)
  • Bioinformatics (20225)
  • Biophysics (9991)
  • Cancer Biology (7717)
  • Cell Biology (11280)
  • Clinical Trials (138)
  • Developmental Biology (6426)
  • Ecology (9930)
  • Epidemiology (2065)
  • Evolutionary Biology (13298)
  • Genetics (9354)
  • Genomics (12566)
  • Immunology (7687)
  • Microbiology (18979)
  • Molecular Biology (7428)
  • Neuroscience (40944)
  • Paleontology (300)
  • Pathology (1226)
  • Pharmacology and Toxicology (2132)
  • Physiology (3146)
  • Plant Biology (6850)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1893)
  • Systems Biology (5306)
  • Zoology (1087)