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The UV RESISTANCE LOCUS 8-Mediated UV-B Response Is Required Alongside CRYPTOCHROME1 For Plant Survival Under Sunlight In The Field

Reinhold Stockenhuber, Reiko Akiyama, Nicolas Tissot, Misako Yamazaki, Michele Wyler, Adriana B. Arongaus, Roman Podolec, Yasuhiro Sato, Stefan Milosavljevic, Alex Widmer, View ORCID ProfileRoman Ulm, View ORCID ProfileKentaro K. Shimizu
doi: https://doi.org/10.1101/2021.12.08.471623
Reinhold Stockenhuber
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland
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Reiko Akiyama
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland
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Nicolas Tissot
2Department of Botany and Plant Biology, Section of Biology, Faculty of Sciences, University of Geneva, Geneva, Switzerland
3Institute of Genetics and Genomics of Geneva (iGE3), University of Geneva, Geneva, Switzerland
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Misako Yamazaki
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland
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Michele Wyler
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland
4Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland
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Adriana B. Arongaus
2Department of Botany and Plant Biology, Section of Biology, Faculty of Sciences, University of Geneva, Geneva, Switzerland
3Institute of Genetics and Genomics of Geneva (iGE3), University of Geneva, Geneva, Switzerland
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Roman Podolec
2Department of Botany and Plant Biology, Section of Biology, Faculty of Sciences, University of Geneva, Geneva, Switzerland
3Institute of Genetics and Genomics of Geneva (iGE3), University of Geneva, Geneva, Switzerland
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Yasuhiro Sato
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland
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Stefan Milosavljevic
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland
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Alex Widmer
5Institute of Integrative Biology, ETH Zurich, Zurich, Switzerland
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Roman Ulm
2Department of Botany and Plant Biology, Section of Biology, Faculty of Sciences, University of Geneva, Geneva, Switzerland
3Institute of Genetics and Genomics of Geneva (iGE3), University of Geneva, Geneva, Switzerland
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  • For correspondence: kentaro.shimizu@ieu.uzh.ch roman.ulm@unige.ch
Kentaro K. Shimizu
1Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland
6Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan
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  • For correspondence: kentaro.shimizu@ieu.uzh.ch roman.ulm@unige.ch
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Abstract

As sessile organisms, plants are subjected to fluctuating sunlight including potentially detrimental ultraviolet-B radiation (UV-B). In Arabidopsis thaliana, experiments under controlled conditions have shown that UV RESISTANCE LOCUS 8 (UVR8) controls photomorphogenic responses for acclimation and tolerance to UV-B; however, its long-term impacts on plant performance remain poorly understood in naturally fluctuating environments. Here we quantified the survival and reproduction of different Arabidopsis mutant genotypes in diverse field and laboratory conditions. We found that uvr8 mutants produced more fruits than wild type in growth chambers with artificial low UV-B conditions but not in natural field conditions. Importantly, independent double mutants of UVR8 and the blue-light photoreceptor gene CRYPTOCHROME 1 (CRY1) in two genetic backgrounds showed a drastic reduction in fitness in the field. UV-B attenuation experiments in field conditions and supplemental UV-B in growth chambers demonstrated that UV-B caused the conditional cry1 uvr8 lethality phenotype. RNA sequencing in different conditions revealed a large number of genes with statistical interaction of UVR8 and CRY1 mutations in the presence of UV-B in the field. Among them, Gene Ontology analysis identified enrichment of categories related to UV-B response, oxidative stress, photoprotection and DNA damage repair. Our study demonstrates the functional importance of the UVR8-mediated response across life stages in natura, which is partially redundant with CRY1, and provides an integral picture of gene expression associated with plant environmental responses under diverse environmental conditions.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 09, 2021.
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The UV RESISTANCE LOCUS 8-Mediated UV-B Response Is Required Alongside CRYPTOCHROME1 For Plant Survival Under Sunlight In The Field
Reinhold Stockenhuber, Reiko Akiyama, Nicolas Tissot, Misako Yamazaki, Michele Wyler, Adriana B. Arongaus, Roman Podolec, Yasuhiro Sato, Stefan Milosavljevic, Alex Widmer, Roman Ulm, Kentaro K. Shimizu
bioRxiv 2021.12.08.471623; doi: https://doi.org/10.1101/2021.12.08.471623
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The UV RESISTANCE LOCUS 8-Mediated UV-B Response Is Required Alongside CRYPTOCHROME1 For Plant Survival Under Sunlight In The Field
Reinhold Stockenhuber, Reiko Akiyama, Nicolas Tissot, Misako Yamazaki, Michele Wyler, Adriana B. Arongaus, Roman Podolec, Yasuhiro Sato, Stefan Milosavljevic, Alex Widmer, Roman Ulm, Kentaro K. Shimizu
bioRxiv 2021.12.08.471623; doi: https://doi.org/10.1101/2021.12.08.471623

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