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Microtubule-associated IQD9 guides cellulose synthase velocity to shape seed mucilage

View ORCID ProfileBo Yang, Gina Stamm, View ORCID ProfileKatharina Bürstenbinder, View ORCID ProfileCătălin Voiniciuc
doi: https://doi.org/10.1101/2021.12.11.472226
Bo Yang
1Independent Junior Research Group–Designer Glycans, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
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Gina Stamm
2Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
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Katharina Bürstenbinder
2Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
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  • For correspondence: Katharina.Buerstenbinder@ipb-halle.de Catalin.Voiniciuc@ipb-halle.de
Cătălin Voiniciuc
1Independent Junior Research Group–Designer Glycans, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
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  • For correspondence: Katharina.Buerstenbinder@ipb-halle.de Catalin.Voiniciuc@ipb-halle.de
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Summary

  • Arabidopsis seeds release large capsules of mucilaginous polysaccharides, which are shaped by an intricate network of cellulosic microfibrils. Cellulose synthase complexes is guided by the microtubule cytoskeleton, but it is unclear which proteins mediate this process in the seed coat epidermis (SCE).

  • Using reverse genetics, we identified IQ67 DOMAIN 9 (IQD9) and KINESIN LIGHT CHAIN-RELATED 1 (KLCR1) as two highly expressed genes during seed development and comprehensively characterized their roles for cell wall polysaccharide biosynthesis and cortical microtubule (MT) organization.

  • Mutations in IQD9 as well as in KLCR1 lead to compact mucilage capsules with aberrant cellulose distribution, which can be rescued by transgene complementation. Double mutant analyses revealed that their closest paralogs (IQD10 and KLCR2, respectively) are not required for mucilage biosynthesis. IQD9 physically interacts with KLCR1 and localizes to cortical MTs to maintain their organization in SCE cells. Similar to the previously identified TONNEAU1 (TON1) RECRUITING MOTIF 4 (TRM4) protein, IQD9 is required to maintain the velocity of cellulose synthases.

  • Our results demonstrate that IQD9, KLCR1 and TRM4 are MT-associated proteins that are required for seed mucilage architecture. This study provides the first direct evidence that members of the IQD, KLCR and TRM families have overlapping roles in guiding the distribution of cell wall polysaccharides. Therefore, SCE cells provide an attractive system to further decipher the complex genetic regulation of polarized cellulose deposition.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • E-Mails

    B.Y. Bo.Yang{at}ipb-halle.de

    G.S. Gina.Stamm{at}ipb-halle.de

    K.B. Katharina.Buerstenbinder{at}ipb-halle.de

    C.V. Catalin.Voiniciuc{at}ipb-halle.de

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted December 12, 2021.
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Microtubule-associated IQD9 guides cellulose synthase velocity to shape seed mucilage
Bo Yang, Gina Stamm, Katharina Bürstenbinder, Cătălin Voiniciuc
bioRxiv 2021.12.11.472226; doi: https://doi.org/10.1101/2021.12.11.472226
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Microtubule-associated IQD9 guides cellulose synthase velocity to shape seed mucilage
Bo Yang, Gina Stamm, Katharina Bürstenbinder, Cătălin Voiniciuc
bioRxiv 2021.12.11.472226; doi: https://doi.org/10.1101/2021.12.11.472226

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