Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The aphid BCR4 structure and activity uncover a new defensin peptide superfamily

Karine Loth, Nicolas Parisot, Françoise Paquet, Catherine Sivignon, Isabelle Rahioui, Mélanie Ribeiro Lopes, Karen Gaget, Gabrielle Duport, Agnès F. Delmas, Vincent Aucagne, Abdelaziz Heddi, Federica Calevro, Pedro da Silva
doi: https://doi.org/10.1101/2021.12.17.472939
Karine Loth
1Centre de Biophysique Moléculaire, CNRS UPR 4301, Orléans, France
2UFR Sciences et Techniques, Université d’Orléans, Orléans, F-45071, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicolas Parisot
3Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Françoise Paquet
1Centre de Biophysique Moléculaire, CNRS UPR 4301, Orléans, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catherine Sivignon
4Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Isabelle Rahioui
4Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mélanie Ribeiro Lopes
3Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karen Gaget
4Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabrielle Duport
4Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Agnès F. Delmas
1Centre de Biophysique Moléculaire, CNRS UPR 4301, Orléans, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vincent Aucagne
1Centre de Biophysique Moléculaire, CNRS UPR 4301, Orléans, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Abdelaziz Heddi
3Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Federica Calevro
4Univ Lyon, INRAE, INSA Lyon, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pedro da Silva
3Univ Lyon, INSA Lyon, INRAE, BF2I, UMR 203, F-69621, Villeurbanne, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: pedro.da-silva@insa-lyon.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Aphids (Hemiptera: Aphidoidea) are among the most injuring insects for agricultural plants and their management is a great challenge in agronomical research. A new class of proteins, called Bacteriocyte-specific Cysteine-Rich (BCR), provides an alternative to chemical insecticides for pest control. BCRs have been initially identified in the pea aphid Acyrthosiphon pisum. They are small disulfide bond-rich proteins expressed exclusively in aphid bacteriocytes, the insect derived cells that host intracellular symbiotic bacteria. Here, we show that one out of the A. pisum BCRs, BCR4, displays an outstanding insecticidal activity against the pea aphid, impairing insect survival and nymphal growth, providing evidence for its potential use as a new biopesticides. Our comparative genomics and phylogenetic analysis indicate that BCRs seem restricted to the aphid lineage. The 3D structure of the BCR4 reveals that this peptide belongs to a yet unknown structural class of peptides and defines a new superfamily of defensins.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted December 17, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The aphid BCR4 structure and activity uncover a new defensin peptide superfamily
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The aphid BCR4 structure and activity uncover a new defensin peptide superfamily
Karine Loth, Nicolas Parisot, Françoise Paquet, Catherine Sivignon, Isabelle Rahioui, Mélanie Ribeiro Lopes, Karen Gaget, Gabrielle Duport, Agnès F. Delmas, Vincent Aucagne, Abdelaziz Heddi, Federica Calevro, Pedro da Silva
bioRxiv 2021.12.17.472939; doi: https://doi.org/10.1101/2021.12.17.472939
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
The aphid BCR4 structure and activity uncover a new defensin peptide superfamily
Karine Loth, Nicolas Parisot, Françoise Paquet, Catherine Sivignon, Isabelle Rahioui, Mélanie Ribeiro Lopes, Karen Gaget, Gabrielle Duport, Agnès F. Delmas, Vincent Aucagne, Abdelaziz Heddi, Federica Calevro, Pedro da Silva
bioRxiv 2021.12.17.472939; doi: https://doi.org/10.1101/2021.12.17.472939

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (4237)
  • Biochemistry (9146)
  • Bioengineering (6785)
  • Bioinformatics (24019)
  • Biophysics (12137)
  • Cancer Biology (9543)
  • Cell Biology (13795)
  • Clinical Trials (138)
  • Developmental Biology (7640)
  • Ecology (11714)
  • Epidemiology (2066)
  • Evolutionary Biology (15517)
  • Genetics (10649)
  • Genomics (14331)
  • Immunology (9491)
  • Microbiology (22856)
  • Molecular Biology (9103)
  • Neuroscience (49027)
  • Paleontology (355)
  • Pathology (1484)
  • Pharmacology and Toxicology (2572)
  • Physiology (3848)
  • Plant Biology (8335)
  • Scientific Communication and Education (1472)
  • Synthetic Biology (2296)
  • Systems Biology (6196)
  • Zoology (1302)