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A comparative meta-analysis of membraneless organelle associated proteins with age related proteome of C. elegans

View ORCID ProfilePritam Mukherjee, View ORCID ProfilePrajnadipta Panda, View ORCID ProfilePrasad Kasturi
doi: https://doi.org/10.1101/2021.12.17.473198
Pritam Mukherjee
1BioX centre, Indian Institute of Technology Mandi Kamand, Himachal Pradesh - 175005, India
2School of Basic Sciences, Indian Institute of Technology Mandi Kamand, Himachal Pradesh - 175005, India
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Prajnadipta Panda
1BioX centre, Indian Institute of Technology Mandi Kamand, Himachal Pradesh - 175005, India
2School of Basic Sciences, Indian Institute of Technology Mandi Kamand, Himachal Pradesh - 175005, India
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Prasad Kasturi
1BioX centre, Indian Institute of Technology Mandi Kamand, Himachal Pradesh - 175005, India
2School of Basic Sciences, Indian Institute of Technology Mandi Kamand, Himachal Pradesh - 175005, India
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  • For correspondence: prasadkasturi@iitmandi.ac.in
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ABSTRACT

Proteome imbalance can lead to protein misfolding and aggregation which is associated with pathologies. Protein aggregation can also be an active, organized process and can be exploited by cells as a survival strategy. In adverse conditions, it is beneficial to deposit the proteins in a condensate rather degrading and resynthesizing. Membrane less organelles (MLOs) are biological condensates formed through liquid–liquid phase separation (LLPS), involving cellular components such as nucleic acids and proteins. LLPS is a regulated process, which when perturbed, can undergo a transition from a physiological liquid condensate to pathological solid-like protein aggregates.

To understand how the MLO-associated proteins (MLO-APs) behave during aging, we performed a comparative meta-analysis with age related proteome of C. elegans. We found that the MLO-APs are highly abundant throughout the lifespan. Interestingly, they are aggregating more in long-lived mutant worms compared to the age matched wildtype worms. GO term analysis revealed that the cell cycle and embryonic development are among the top enriched processes in addition to RNA metabolism RNP components. Considering antagonistic pleotropic nature of these developmental genes and post mitotic status of C. elegans, we assume that these proteins phase transit during post development. As the organism ages, these MLO-APs either mature to become more insoluble or dissolve in uncontrolled manner. However, in the long-lived daf-2 mutant worms, the MLOs may attain protective states due to enhanced proteostasis components and altered metabolism that eventually make these worms more protected.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted December 18, 2021.
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A comparative meta-analysis of membraneless organelle associated proteins with age related proteome of C. elegans
Pritam Mukherjee, Prajnadipta Panda, Prasad Kasturi
bioRxiv 2021.12.17.473198; doi: https://doi.org/10.1101/2021.12.17.473198
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A comparative meta-analysis of membraneless organelle associated proteins with age related proteome of C. elegans
Pritam Mukherjee, Prajnadipta Panda, Prasad Kasturi
bioRxiv 2021.12.17.473198; doi: https://doi.org/10.1101/2021.12.17.473198

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