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Remdesivir, Molnupiravir and Nirmatrelvir remain active against SARS-CoV-2 Omicron and other variants of concern

View ORCID ProfileLaura Vangeel, View ORCID ProfileSteven De Jonghe, View ORCID ProfilePiet Maes, Bram Slechten, Joren Raymenants, View ORCID ProfileEmmanuel André, View ORCID ProfileJohan Neyts, View ORCID ProfileDirk Jochmans
doi: https://doi.org/10.1101/2021.12.27.474275
Laura Vangeel
1KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium
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  • ORCID record for Laura Vangeel
Steven De Jonghe
1KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium
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Piet Maes
2KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium
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Bram Slechten
3University Hospitals Leuven, Department of Laboratory Medicine, Leuven, Belgium
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Joren Raymenants
3University Hospitals Leuven, Department of Laboratory Medicine, Leuven, Belgium
4KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Clinical Bacteriology and Mycology, Leuven, Belgium
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Emmanuel André
3University Hospitals Leuven, Department of Laboratory Medicine, Leuven, Belgium
4KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Clinical Bacteriology and Mycology, Leuven, Belgium
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Johan Neyts
1KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium
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  • For correspondence: dirk.jochmans@kuleuven.be johan.neyts@kuleuven.be
Dirk Jochmans
1KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium
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  • For correspondence: dirk.jochmans@kuleuven.be johan.neyts@kuleuven.be
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Abstract

The in vitro effect of GS-441524, remdesivir, EIDD-1931, molnupiravir and nirmatrelvir against the various SARS-CoV-2 VOCs, including Omicron, was determined. VeroE6-GFP cells were pre-treated overnight with serial dilutions of the compounds before infection. The number of fluorescent pixels of GFP signal, determined by high-content imaging on day 4 post-infection, was used as read-out, and the EC50 of each compound on a viral isolate of each VOC was calculated. These experiments were performed in the presence of the Pgp-inhibitor CP-100356 in order to limit compound efflux. A SARS-CoV-2 strain grown from the first Belgian patient sample was used as ancestral strain. All the other isolates were obtained from patients in Belgium as well.

Our results indicate that GS-441524, remdesivir, EIDD-1931, molnupiravir and nirmatrelvir retain their activity against the VOCs Alpha, Beta, Gamma, Delta and Omicron. This is in accordance with the observation that the target proteins of these antivirals are highly conserved.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted December 28, 2021.
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Remdesivir, Molnupiravir and Nirmatrelvir remain active against SARS-CoV-2 Omicron and other variants of concern
Laura Vangeel, Steven De Jonghe, Piet Maes, Bram Slechten, Joren Raymenants, Emmanuel André, Johan Neyts, Dirk Jochmans
bioRxiv 2021.12.27.474275; doi: https://doi.org/10.1101/2021.12.27.474275
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Remdesivir, Molnupiravir and Nirmatrelvir remain active against SARS-CoV-2 Omicron and other variants of concern
Laura Vangeel, Steven De Jonghe, Piet Maes, Bram Slechten, Joren Raymenants, Emmanuel André, Johan Neyts, Dirk Jochmans
bioRxiv 2021.12.27.474275; doi: https://doi.org/10.1101/2021.12.27.474275

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