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Cortical glutamatergic projection neuron types contribute to distinct functional subnetworks

View ORCID ProfileHemanth Mohan, Xu An, Hideki Kondo, Shengli Zhao, Katherine S. Matho, Simon Musall, Partha Mitra, View ORCID ProfileZ. Josh Huang
doi: https://doi.org/10.1101/2021.12.30.474537
Hemanth Mohan
1Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
3Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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  • ORCID record for Hemanth Mohan
Xu An
1Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
3Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Hideki Kondo
3Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Shengli Zhao
1Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
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Katherine S. Matho
3Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Simon Musall
3Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
4Institute of Biological information Processing, Forschungszentrum Julich, Julich, Germany
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Partha Mitra
3Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Z. Josh Huang
1Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
2Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
3Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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  • For correspondence: josh.huang@duke.edu
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Abstract

The cellular basis of cerebral cortex functional architecture remains not well understood. A major challenge is to monitor and decipher neural network dynamics across broad cortical areas yet with projection neuron (PN)-type resolution in real time during behavior. Combining genetic targeting and wide-field imaging, we monitored activity dynamics of subcortical-projecting (PTFezf2) and intratelencephalic-projecting (ITPlxnD1) types across dorsal cortex of mice during different brain states and behaviors. ITPlxnD1 and PTFezf2 neurons showed distinct activation patterns during wakeful resting, spontaneous movements, and upon sensory stimulation. Distinct ITPlxnD1 and PTFezf2 subnetworks were dynamically tuned to different sensorimotor components of a naturalistic feeding behavior, and optogenetic inhibition of subnetwork nodes disrupted specific components of this behavior. Lastly, ITPlxnD1 and PTFezf2 projection patterns are consistent with their subnetwork activation patterns. Our results show that, in addition to the concept of columnar organization, dynamic areal and PN type-specific subnetworks are a key feature of cortical functional architecture linking microcircuit components with global brain networks.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 08, 2022.
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Cortical glutamatergic projection neuron types contribute to distinct functional subnetworks
Hemanth Mohan, Xu An, Hideki Kondo, Shengli Zhao, Katherine S. Matho, Simon Musall, Partha Mitra, Z. Josh Huang
bioRxiv 2021.12.30.474537; doi: https://doi.org/10.1101/2021.12.30.474537
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Cortical glutamatergic projection neuron types contribute to distinct functional subnetworks
Hemanth Mohan, Xu An, Hideki Kondo, Shengli Zhao, Katherine S. Matho, Simon Musall, Partha Mitra, Z. Josh Huang
bioRxiv 2021.12.30.474537; doi: https://doi.org/10.1101/2021.12.30.474537

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