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Bioprinting the Tumor Microenvironment with an Upgraded Consumer Stereolithographic 3D Printer

Louise Breideband, Kaja N. Wächtershäuser, Levin Hafa, Konstantin Wieland, Achilleas Frangakis, Ernst H. K. Stelzer, View ORCID ProfileFrancesco Pampaloni
doi: https://doi.org/10.1101/2021.12.30.474546
Louise Breideband
1Biological Sciences (IZN), FCAM, BMLS, Goethe-Universität Frankfurt am Main, Max-von-Laue-Straße 15, DE-60439 Frankfurt am Main, Germany
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Kaja N. Wächtershäuser
1Biological Sciences (IZN), FCAM, BMLS, Goethe-Universität Frankfurt am Main, Max-von-Laue-Straße 15, DE-60439 Frankfurt am Main, Germany
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Levin Hafa
1Biological Sciences (IZN), FCAM, BMLS, Goethe-Universität Frankfurt am Main, Max-von-Laue-Straße 15, DE-60439 Frankfurt am Main, Germany
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Konstantin Wieland
2FCEM, BMLS, Goethe-Universität Frankfurt am Main, Max-von-Laue-Straße 15, DE-60439 Frankfurt am Main, Germany
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Achilleas Frangakis
2FCEM, BMLS, Goethe-Universität Frankfurt am Main, Max-von-Laue-Straße 15, DE-60439 Frankfurt am Main, Germany
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Ernst H. K. Stelzer
1Biological Sciences (IZN), FCAM, BMLS, Goethe-Universität Frankfurt am Main, Max-von-Laue-Straße 15, DE-60439 Frankfurt am Main, Germany
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Francesco Pampaloni
1Biological Sciences (IZN), FCAM, BMLS, Goethe-Universität Frankfurt am Main, Max-von-Laue-Straße 15, DE-60439 Frankfurt am Main, Germany
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  • ORCID record for Francesco Pampaloni
  • For correspondence: fpampalo@bio.uni-frankfurt.de
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Abstract

A widespread application of three-dimensional (3D) bioprinting in basic and translational research requires the accessibility to affordable printers able to produce physiologically relevant tissue models. To facilitate the use of bioprinting as a standard technique in biology, an open- source device based on a consumer-grade 3D stereolithographic (SL) printer was developed. This SL bioprinter can produce complex constructs that preserve cell viability and recapitulate the physiology of tissues. The detailed documentation of the modifications apported to the printer as well as a throughout performance analysis allow for a straightforward adoption of the device in other labs and its customization for specific applications. Given the low cost, several modified bioprinters could be simultaneously operated for a highly parallelized tissue production. To showcase the capability of the bioprinter, we produced constructs consisting of patient- derived cholangiocarcinoma organoids encapsulated in a gelatin methacrylate (GelMA)/polyethylene glycol diacrylate (PEGDA) hydrogel. A thorough characterization of different GelMA/PEGDA ratios revealed that the mechanical properties of the bioprinted tumor model can be accurately fine-tuned to mimic a specific tumor micro-environment. Immunofluorescence and gene expression analyses of tumor markers confirmed that the bioprinted synthetic hydrogel provides a flexible and adequate replacement of animal-derived reconstituted extracellular matrix.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://github.com/LouiseBreide/BreidebandSupportingInformation/blob/main/Breideband_3Dbioprinter_Supplementary.pdf

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 31, 2021.
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Bioprinting the Tumor Microenvironment with an Upgraded Consumer Stereolithographic 3D Printer
Louise Breideband, Kaja N. Wächtershäuser, Levin Hafa, Konstantin Wieland, Achilleas Frangakis, Ernst H. K. Stelzer, Francesco Pampaloni
bioRxiv 2021.12.30.474546; doi: https://doi.org/10.1101/2021.12.30.474546
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Bioprinting the Tumor Microenvironment with an Upgraded Consumer Stereolithographic 3D Printer
Louise Breideband, Kaja N. Wächtershäuser, Levin Hafa, Konstantin Wieland, Achilleas Frangakis, Ernst H. K. Stelzer, Francesco Pampaloni
bioRxiv 2021.12.30.474546; doi: https://doi.org/10.1101/2021.12.30.474546

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