Abstract
Mycobacterium abscessus (Mabs) drives life-shortening mortality in cystic fibrosis (CF) patients, primarily because of its resistance to chemotherapeutic agents. Both our knowledge on and models to investigate the host and bacterial determinants that drive Mabs pathology in CF patients remain rudimentary. Here, we evaluated whether the lung organoid technology from CF patients is appropriate for modelling Mabs infection and whether antioxidant treatment is a candidate therapeutic approach in the context of CF disease. We derived airway organoids (AOs) from lung biopsy of a CF patient and characterized these AO by assessing CF transmembrane conductance regulator (CFTR) function, mucus and reactive oxygen species (ROS) production, lipid peroxidation, and cell death. We microinjected smooth (S-) or rough (R-)Mabs in the lumen of AOs to evaluate its fitness, responses of AOs to infection, and treatment efficacy by colony forming unit assay, qPCR and microscopy. We show that CF patient-derived AOs exhibited low residual CFTR function, enhanced mucus accumulation, and increased oxidative stress, lipid peroxidation, and cell death at basal state. While in AOs, S Mabs formed biofilm, R Mabs formed cord serpentines and displayed a higher virulence. S and R Mabs replicated more efficiently in CF AOs than in AOs derived from healthy lung. Pharmacological activation of antioxidant pathways resulted in better control of Mabs growth. In conclusion, we have established CF patient-derived AOs as a suitable human system to decipher mechanisms of CF-enhanced respiratory infection by Mabs and confirmed antioxidant approaches as a potential host-directed strategy to improve Mabs infection control.
Competing Interest Statement
H.C is inventor on patents related to organoid technology. Other authors declare no conflict of interest, his full disclosure is given at https://www.uu.nl/staff/JCClevers/.
Footnotes
Grant support This project has been funded by grants from “Vaincre La Mucoviscidose” and “Grégory Lemarchal” foundations (N°RF20210502852/1/1/48) to CC, from the “Fondation pour la Recherche Médicale” (FRM) and ERC StG (INFLAME) to EM. SALI was funded by “Vaincre La Mucoviscidose” PhD fellowship (N°RF20210502852/1/1/48). This work was also supported by grants from CNRS (IEA 300134) to CC, Campus France PHC Van Gogh (40577ZE) to GL-V, ZonMW 3R’s (114021005) to PJP, the Nuffic Van Gogh Programme (VGP.17/10 to NI), and by the LINK program from the Province of Limburg, the Netherlands. TA, VM and LB were funded by “La Région Languedoc-Roussillon” (N° DRTE/RSS - ESR_R&S_DF-000061-2018-003268).
Funders had no interference in the conduct of the project.
Competing Interest Statement: H.C is inventor on patents related to organoid technology. Other authors declare no conflict of interest, his full disclosure is given at https://www.uu.nl/staff/JCClevers/.