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Conserved meiotic mechanisms in the cnidarian Clytia hemisphaerica revealed by Spo11 knockout

View ORCID ProfileCatriona Munro, Hugo Cadis, Sophie Pagnotta, View ORCID ProfileEvelyn Houliston, View ORCID ProfileJean-René Huynh
doi: https://doi.org/10.1101/2022.01.05.475076
Catriona Munro
1Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, Université PSL, Paris, France
2Sorbonne Université, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), 06230, France
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Hugo Cadis
1Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, Université PSL, Paris, France
2Sorbonne Université, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), 06230, France
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Sophie Pagnotta
3Centre Commun de Microscopie Appliquée, Université Nice Côte d’Azur, Parc Valrose, 06108 Nice, France
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Evelyn Houliston
2Sorbonne Université, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), 06230, France
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Jean-René Huynh
1Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, Université PSL, Paris, France
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  • For correspondence: jean-rene.huynh@college-de-france.fr
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Abstract

During meiosis, DNA recombination allows the shuffling of genetic information between the maternal and paternal chromosomes. Recombination is initiated by double strand breaks (DSBs) catalyzed by the conserved enzyme Spo11. How this crucial event is connected to other meiotic processes is surprisingly variable depending on the species. Here, we knocked down Spo11 by CRISPR in the jellyfish Clytia hemisphaerica, belonging to Cnidaria, the sister group to Bilateria (where classical animal models are found). Spo11 mutants fail to assemble synaptonemal complexes and chiasmata, and in consequence homologous chromosome pairs disperse during oocyte growth, creating aneuploid but fertilizable eggs that develop into viable larvae. Clytia thus shares an ancient eukaryotic dependence of synapsis and chromosome segregation on Spo11-generated DSBs, and provides new evolutionary perspectives on meiosis regulation.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 20, 2022.
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Conserved meiotic mechanisms in the cnidarian Clytia hemisphaerica revealed by Spo11 knockout
Catriona Munro, Hugo Cadis, Sophie Pagnotta, Evelyn Houliston, Jean-René Huynh
bioRxiv 2022.01.05.475076; doi: https://doi.org/10.1101/2022.01.05.475076
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Conserved meiotic mechanisms in the cnidarian Clytia hemisphaerica revealed by Spo11 knockout
Catriona Munro, Hugo Cadis, Sophie Pagnotta, Evelyn Houliston, Jean-René Huynh
bioRxiv 2022.01.05.475076; doi: https://doi.org/10.1101/2022.01.05.475076

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