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Multi-micron crisscross structures from combinatorially assembled DNA-origami slats

Christopher M. Wintersinger, Dionis Minev, Anastasia Ershova, Hiroshi M. Sasaki, Gokul Gowri, Jonathan F. Berengut, F. Eduardo Corea-Dilbert, Peng Yin, William M. Shih
doi: https://doi.org/10.1101/2022.01.06.475243
Christopher M. Wintersinger
1Wyss Institute for Biologically Inspired Engineering at Harvard University
2Harvard John A. Paulson School of Engineering and Applied Sciences
3Department of Cancer Biology, Dana-Farber Cancer Institute
4Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
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Dionis Minev
1Wyss Institute for Biologically Inspired Engineering at Harvard University
3Department of Cancer Biology, Dana-Farber Cancer Institute
4Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
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Anastasia Ershova
1Wyss Institute for Biologically Inspired Engineering at Harvard University
3Department of Cancer Biology, Dana-Farber Cancer Institute
4Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
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Hiroshi M. Sasaki
1Wyss Institute for Biologically Inspired Engineering at Harvard University
6Department of Systems Biology, Harvard Medical School
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Gokul Gowri
1Wyss Institute for Biologically Inspired Engineering at Harvard University
3Department of Cancer Biology, Dana-Farber Cancer Institute
4Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
6Department of Systems Biology, Harvard Medical School
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Jonathan F. Berengut
1Wyss Institute for Biologically Inspired Engineering at Harvard University
3Department of Cancer Biology, Dana-Farber Cancer Institute
5University of New South Wales Sydney
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F. Eduardo Corea-Dilbert
3Department of Cancer Biology, Dana-Farber Cancer Institute
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Peng Yin
1Wyss Institute for Biologically Inspired Engineering at Harvard University
6Department of Systems Biology, Harvard Medical School
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William M. Shih
1Wyss Institute for Biologically Inspired Engineering at Harvard University
3Department of Cancer Biology, Dana-Farber Cancer Institute
4Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
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  • For correspondence: William_Shih@dfci.harvard.edu
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Abstract

Living systems achieve robust self-assembly across length scales. Meanwhile, nanofabrication strategies such as DNA origami have enabled robust self-assembly of submicron-scale shapes.However, erroneous and missing linkages restrict the number of unique origami that can be practically combined into a single supershape. We introduce crisscross polymerization of DNA-origami slats for strictly seed-dependent growth of custom multi-micron shapes with user-defined nanoscale surface patterning. Using a library of ~2000 strands that can be combinatorially assembled to yield any of ~1e48 distinct DNA origami slats, we realize five-gigadalton structures composed of >1000 uniquely addressable slats, and periodic structures incorporating >10,000 slats. Thus crisscross growth provides a generalizable route for prototyping and scalable production of devices integrating thousands of unique components that each are sophisticated and molecularly precise.

One-sentence summary Crisscross polymerization of DNA-origami slats can yield micron-scale structures with uniquely addressable nanoscale features.

Competing Interest Statement

A patent (PCT/US2017/045013) has been filed based on this work.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted January 07, 2022.
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Multi-micron crisscross structures from combinatorially assembled DNA-origami slats
Christopher M. Wintersinger, Dionis Minev, Anastasia Ershova, Hiroshi M. Sasaki, Gokul Gowri, Jonathan F. Berengut, F. Eduardo Corea-Dilbert, Peng Yin, William M. Shih
bioRxiv 2022.01.06.475243; doi: https://doi.org/10.1101/2022.01.06.475243
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Multi-micron crisscross structures from combinatorially assembled DNA-origami slats
Christopher M. Wintersinger, Dionis Minev, Anastasia Ershova, Hiroshi M. Sasaki, Gokul Gowri, Jonathan F. Berengut, F. Eduardo Corea-Dilbert, Peng Yin, William M. Shih
bioRxiv 2022.01.06.475243; doi: https://doi.org/10.1101/2022.01.06.475243

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