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Cut-and-Paste DNA Insertion with Engineered Type V-K CRISPR-associated Transposases

View ORCID ProfileConnor J. Tou, View ORCID ProfileBenno Orr, View ORCID ProfileBenjamin P. Kleinstiver
doi: https://doi.org/10.1101/2022.01.07.475005
Connor J. Tou
1Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, 02114, USA
2Department of Pathology, Massachusetts General Hospital, Boston, MA, 02114, USA
3Biological Engineering Program, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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Benno Orr
1Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, 02114, USA
2Department of Pathology, Massachusetts General Hospital, Boston, MA, 02114, USA
4Biological and Biomedical Sciences Program, Harvard University, Boston, MA, 02115, USA
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Benjamin P. Kleinstiver
1Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, 02114, USA
2Department of Pathology, Massachusetts General Hospital, Boston, MA, 02114, USA
5Department of Pathology, Harvard Medical School, Boston, MA, 02115, USA
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  • For correspondence: bkleinstiver@mgh.harvard.edu
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Article Information

doi 
https://doi.org/10.1101/2022.01.07.475005
History 
  • January 9, 2022.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Connor J. Tou1,2,3,
  2. Benno Orr1,2,4 and
  3. Benjamin P. Kleinstiver1,2,5,*
  1. 1Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, 02114, USA
  2. 2Department of Pathology, Massachusetts General Hospital, Boston, MA, 02114, USA
  3. 3Biological Engineering Program, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
  4. 4Biological and Biomedical Sciences Program, Harvard University, Boston, MA, 02115, USA
  5. 5Department of Pathology, Harvard Medical School, Boston, MA, 02115, USA
  1. ↵*correspondence to bkleinstiver{at}mgh.harvard.edu
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Posted January 09, 2022.
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Cut-and-Paste DNA Insertion with Engineered Type V-K CRISPR-associated Transposases
Connor J. Tou, Benno Orr, Benjamin P. Kleinstiver
bioRxiv 2022.01.07.475005; doi: https://doi.org/10.1101/2022.01.07.475005
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Cut-and-Paste DNA Insertion with Engineered Type V-K CRISPR-associated Transposases
Connor J. Tou, Benno Orr, Benjamin P. Kleinstiver
bioRxiv 2022.01.07.475005; doi: https://doi.org/10.1101/2022.01.07.475005

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