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Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation

Jaclyn Camuglia, Soline Chanet, Adam C Martin
doi: https://doi.org/10.1101/2022.01.07.475359
Jaclyn Camuglia
1Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139
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Soline Chanet
2Center for Interdisciplinary Research in Biology, Collège de France, 11 Pl. Marcelin Berthelot, 75231 Paris
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Adam C Martin
1Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139
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  • For correspondence: acmartin@mit.edu
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Abstract

Spindle orientation is often achieved by a complex of Pins/LGN, Mud/NuMa, Gαi, and Dynein, which interacts with astral microtubules to rotate the spindle. Cortical Pins/LGN recruitment serves as a critical step in this process. Here, we identify Pins-mediated planar cell polarized divisions in several of the mitotic domains of the early Drosophila embryo. We found that neither planar cell polarity pathways nor planar polarized myosin localization determined division orientation; instead, our findings strongly suggest that Pins planar polarity and force generated from mesoderm invagination are important. Disrupting Pins polarity via overexpression of a myristoylated version of Pins caused randomized division angles. We found that disrupting forces through chemical inhibitors, laser ablation, and depletion of an adherens junction protein disrupted Pins planar polarity and spindle orientation. Furthermore, snail depletion, which abrogates ventral furrow forces, disrupted Pins polarization and spindle orientation, suggesting that morphogenetic movements and resulting forces transmitted through the tissue can polarize Pins and orient division. Thus, morphogenetic forces associated with mesoderm invagination result in planar polarized Pins to mediate division orientation at a distant region of the embryo during morphogenesis. To our knowledge, this is the first in vivo example where mechanical force has been shown to polarize Pins to mediate division orientation.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted January 07, 2022.
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Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation
Jaclyn Camuglia, Soline Chanet, Adam C Martin
bioRxiv 2022.01.07.475359; doi: https://doi.org/10.1101/2022.01.07.475359
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Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation
Jaclyn Camuglia, Soline Chanet, Adam C Martin
bioRxiv 2022.01.07.475359; doi: https://doi.org/10.1101/2022.01.07.475359

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