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SH3Ps recruit auxilin-like vesicle uncoating factors into clathrin-mediated endocytosis

Maciek Adamowski, Ivana Matijević, Madhumitha Narasimhan, Jiří Friml
doi: https://doi.org/10.1101/2022.01.07.475403
Maciek Adamowski
Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria
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Ivana Matijević
Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria
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Madhumitha Narasimhan
Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria
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Jiří Friml
Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria
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  • For correspondence: jiri.friml@ist.ac.at
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Abstract

Clathrin-mediated endocytosis (CME) is an essential process of cellular cargo uptake operating in all eukaryotes. In animal and yeast, CME involves BAR-SH3 domain proteins, endophilins and amphiphysins, which function at the conclusion of CME to recruit factors for vesicle scission and uncoating. Arabidopsis thaliana contains BAR-SH3 domain proteins SH3P1-3, but their role is poorly understood. We identify SH3P1-3 as functional homologues of endophilin/amphiphysin. SH3P1-3 bind to discrete foci at the plasma membrane (PM), and colocalization indicates late recruitment of SH3P2 to a subset of clathrin-coated pits. PM recruitment pattern of SH3P2 is nearly identical to its interactor, a putative vesicle uncoating factor AUXILIN-LIKE1, and SH3P1-3 are required for most of AUXILIN-LIKE1 PM binding. This indicates a plant-specific modification of CME, where BAR-SH3 proteins recruit auxilin-like uncoating factors, rather than the uncoating phosphatases synaptojanins. Furthermore, we identify an unexpected redundancy between SH3P1-3 and a plant-specific endocytic adaptor, TPLATE complex, showing a contribution of SH3P1-3 to gross CME.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted January 09, 2022.
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SH3Ps recruit auxilin-like vesicle uncoating factors into clathrin-mediated endocytosis
Maciek Adamowski, Ivana Matijević, Madhumitha Narasimhan, Jiří Friml
bioRxiv 2022.01.07.475403; doi: https://doi.org/10.1101/2022.01.07.475403
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SH3Ps recruit auxilin-like vesicle uncoating factors into clathrin-mediated endocytosis
Maciek Adamowski, Ivana Matijević, Madhumitha Narasimhan, Jiří Friml
bioRxiv 2022.01.07.475403; doi: https://doi.org/10.1101/2022.01.07.475403

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