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Sub-second striatal dopamine dynamics assessed by simultaneous fast-scan cyclic voltammetry and fluorescence biosensor

Armando G. Salinas, Jeong O. Lee, Shana M. Augustin, Shiliang Zhang, Tommaso Patriarchi, Lin Tian, Marisela Morales, Yolanda Mateo, David M. Lovinger
doi: https://doi.org/10.1101/2022.01.09.475513
Armando G. Salinas
1Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA
2Department of Bioengineering, George Mason University, Fairfax, VA, USA
7Department of Pharmacology, Toxicology & Neuroscience, Louisiana State University Health Sciences Center – Shreveport, Shreveport, LA, USA
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Jeong O. Lee
1Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA
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Shana M. Augustin
1Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA
8Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA
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Shiliang Zhang
3Confocal and Electron Microscopy Core, National Institute on Drug Abuse, Baltimore, MD, USA
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Tommaso Patriarchi
4Department of Biochemistry and Molecular Medicine, University of California at Davis, Davis, CA, USA
5Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland
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Lin Tian
4Department of Biochemistry and Molecular Medicine, University of California at Davis, Davis, CA, USA
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Marisela Morales
6Neuronal Networks Section, Integrative Neuroscience Research Branch, National Institute on Drug Abuse, Baltimore, MD, USA
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Yolanda Mateo
1Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA
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David M. Lovinger
1Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA
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  • For correspondence: lovindav@mail.nih.gov
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Abstract

Fast-scan cyclic voltammetry (FSCV) is an electrochemical method used to detect dopamine on a subsecond time scale. Recordings using FSCV in freely behaving animals revolutionized the study of behaviors associated with motivation and learning. Despite this advance, FSCV cannot distinguish between catecholamines, which limits its use to brain regions where dopamine is the predominant neurotransmitter. It has also been difficult to detect dopamine in vivo in some striatal subregions with FSCV. Recently, fluorescent biosensors for dopamine were developed, allowing for discrimination between catecholamines. However, the performance of these biosensors relative to FSCV has not been determined. Thus, we compared fluorescent photometry responses of the dopamine biosensor, dLight, with FSCV. We also used dLight photometry to assess changes in tonic and phasic dopamine, which has not been possible with FSCV. Finally, we examined dopamine dynamics during Pavlovian conditioning in striatal subregions, including the dorsolateral striatum where dopamine measurements are challenging with FSCV.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted January 11, 2022.
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Sub-second striatal dopamine dynamics assessed by simultaneous fast-scan cyclic voltammetry and fluorescence biosensor
Armando G. Salinas, Jeong O. Lee, Shana M. Augustin, Shiliang Zhang, Tommaso Patriarchi, Lin Tian, Marisela Morales, Yolanda Mateo, David M. Lovinger
bioRxiv 2022.01.09.475513; doi: https://doi.org/10.1101/2022.01.09.475513
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Sub-second striatal dopamine dynamics assessed by simultaneous fast-scan cyclic voltammetry and fluorescence biosensor
Armando G. Salinas, Jeong O. Lee, Shana M. Augustin, Shiliang Zhang, Tommaso Patriarchi, Lin Tian, Marisela Morales, Yolanda Mateo, David M. Lovinger
bioRxiv 2022.01.09.475513; doi: https://doi.org/10.1101/2022.01.09.475513

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