High-throughput direct screening of restriction endonuclease using microfluidic fluorescence-activated drop sorter based on SOS response in E. coli

Yizhe Zhang; Jeremy J. Agresti; Yu Zheng; David A. Weitz

doi:10.1101/2022.01.09.475563

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High-throughput direct screening of restriction endonuclease using microfluidic fluorescence-activated drop sorter based on SOS response in E. coli

Yizhe Zhang, Jeremy J. Agresti, Yu Zheng, View ORCID ProfileDavid A. Weitz

doi: https://doi.org/10.1101/2022.01.09.475563

Yizhe Zhang

1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA

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Jeremy J. Agresti

2John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA

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Yu Zheng

3New England BioLabs, Inc., Ipswich, MA 01938, USA

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David A. Weitz

2John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA

4Department of Physics, Harvard University, Cambridge, MA 02138, USA

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ORCID record for David A. Weitz
For correspondence: weitz@seas.harvard.edu

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Posted January 11, 2022.

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High-throughput direct screening of restriction endonuclease using microfluidic fluorescence-activated drop sorter based on SOS response in E. coli

Yizhe Zhang, Jeremy J. Agresti, Yu Zheng, David A. Weitz

bioRxiv 2022.01.09.475563; doi: https://doi.org/10.1101/2022.01.09.475563

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High-throughput direct screening of restriction endonuclease using microfluidic fluorescence-activated drop sorter based on SOS response in E. coli

Yizhe Zhang, Jeremy J. Agresti, Yu Zheng, David A. Weitz

bioRxiv 2022.01.09.475563; doi: https://doi.org/10.1101/2022.01.09.475563

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