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Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions

Patrick Renner, Michael Crone, Matthew Kornas, KimAnh T. Pioli, View ORCID ProfilePeter D. Pioli
doi: https://doi.org/10.1101/2022.01.10.475671
Patrick Renner
cWestern Michigan University Homer Stryker M.D. School of Medicine, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
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Michael Crone
cWestern Michigan University Homer Stryker M.D. School of Medicine, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
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Matthew Kornas
cWestern Michigan University Homer Stryker M.D. School of Medicine, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
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KimAnh T. Pioli
aCenter for Immunobiology, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
bDepartment of Investigative Medicine, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
cWestern Michigan University Homer Stryker M.D. School of Medicine, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
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Peter D. Pioli
aCenter for Immunobiology, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
bDepartment of Investigative Medicine, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
cWestern Michigan University Homer Stryker M.D. School of Medicine, 1000 Oakland Drive, Kalamazoo, MI, 49008 United States
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  • ORCID record for Peter D. Pioli
  • For correspondence: peter.pioli@med.wmich.edu petepioli@gmail.com
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Posted January 11, 2022.
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Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions
Patrick Renner, Michael Crone, Matthew Kornas, KimAnh T. Pioli, Peter D. Pioli
bioRxiv 2022.01.10.475671; doi: https://doi.org/10.1101/2022.01.10.475671
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Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions
Patrick Renner, Michael Crone, Matthew Kornas, KimAnh T. Pioli, Peter D. Pioli
bioRxiv 2022.01.10.475671; doi: https://doi.org/10.1101/2022.01.10.475671

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