Abstract
Study Design Preclinical animal study
Objective Evaluation of the degenerative progression resulting from either a partial- or full-width injury to the mouse lumbar intervertebral disc (IVD) using contrast-enhanced micro-computed tomography and histological analyses. We utilized a lateral-retroperitoneal surgical approach to access the lumbar IVD, and the injuries to the IVD were induced by either incising one side of the annulus fibrosus or puncturing both sides of the annulus fibrosus. The full-width injury caused dramatic reduction in nucleus pulposus hydration and significant degeneration. A partial-width injury produces localized deterioration around the annulus fibrosus site that resulted in local tissue remodeling without gross degeneration to the IVD.
Methods Female C57BL/6J mice of 3-4 months age were used in this study. They were divided into three groups to undergo a partial-width, full-width, or sham injuries. The L5/L6 and L6/S1 lumbar IVDs were surgically exposed using a lateral-retroperitoneal approach. The L6/S1 IVDs were injured using either a surgical scalpel (partial-width) or a 33G needle (full-width), with the L5/L6 serving as an internal control. These animals were allowed to recover and then sacrificed at 2-, 4-, or 8-weeks post-surgery. The IVDs were assessed for degeneration using contrast-enhanced microCT (CEµCT) and histological analysis.
Results The high-resolution 3D evaluation of the IVD confirmed that the respective injuries localized within one side of the annulus fibrosus or spanned the full width of the IVD. The full-width injury caused deteriorations in the nucleus pulposus after 2 weeks that culminated in significant degeneration at 8 weeks, while the partial width injury caused localized disruptions that remained limited to the annulus fibrosus.
Conclusion The use of CEμCT revealed distinct IVD degeneration profiles resulting from partial- and full-width injuries. The partial width injury may serve as a better model for IVD degeneration resulting from localized annulus fibrosus injuries in humans.
Introduction
Mouse models are often used for the preclinical validation of treatments and therapeutic candidates. Mice offer particular advantages of relatively easy maintenance, year-round breeding with a short gestation period, delivery of large litters, and inbred tolerance1. Furthermore, the ability to manipulate the mouse genome enables mechanistic studies with greater biological precision than larger mammalian models including rats, rabbits, and pigs1. Moreover, the mouse lumbar intervertebral disc (IVD) provides close geometric and microstructural semblance to the human lumbar IVD compared to other preclinical animal models2. While the mouse lumbar IVD exhibits age-related degeneration3,4, an injury is often utilized to reproduce the inflammatory conditions and accelerate the IVD’s degenerative cascade5,6.
To create a targeted injury to the IVD, surgical exposure of the IVD is required. These commonly involve the posterior-lateral, transperitoneal, and anterior/lateral retroperitoneal surgical access. Of these, the retroperitoneal approach offers multiple advantages: (1) minimal damage to major organs, vessels, and musculature; (2) access to multiple levels with a relatively small incision; and (3) visual availability of the target tissue under microscopy. Moss et al. demonstrated the efficacy and reproducibility of the retroperitoneal approach in rabbits 7. Exposing the lumbar IVD enables the ability to create a targeted, directed injury for the investigation of the subsequent degenerative process 8–10. Masuda et al. and Sobajima et al. described the rabbit annulus fibrosus injury model where the injury was confined to the annulus fibrosus that caused slow progressive degeneration of the IVD over 8 weeks. The phenotype and the progressive nature of this model recapitulates the human disease, and may be better suited to explore therapies that leverage biological regenerative strategies. In contrast, more damaging approaches that injures both the annulus fibrosus and nucleus pulposus produce rapid and severe course of degeneration 7,11. There are several studies describing IVD degeneration using the lumbosacral IVD injury in the mouse model12–14. In the mouse IVD where the average disc height is approximately 300-400 microns, an injury by needle puncture produces damage to 55-90% across the height and 15-40% across the width of the IVD2,11, representing significant trauma that is atypical in human IVDs. Moreover, these procedures involve damage and injury to the nucleus pulposus, such as with a complete puncture to the IVD, with no possibility to decouple the contributions of the annulus fibrosus and the nucleus pulposus toward the ensuing degenerative cascade. Piazza and coauthors have shown that unilateral (half-width) and bilateral (full-width) injuries in the tail IVD results in unique degenerative trajectories, but this has not been investigated in the mouse lumbar spine. We thus sought to compare the degenerative profiles of IVDs after partial- and full-width injuries in the mouse lumbar spine. In order to evaluate the degeneration of the IVD in a spatially robust manner, we utilized contrast-enhanced microCT15,16, in addition to histological grading, to quantify the changes in structure and composition at 2-, 4- and 8-weeks after surgery.
Materials and Methods
Animal Preparation
All procedures were performed following Washington University School of Medicine IACUC approval. Female C57BL/6J mice of 3-4 months age were used (BW: 20 – 25 g). They were housed under standard animal husbandry conditions (in a temperature-controlled [21 ± 1°C] room with normal 12-hr light/dark cycles). These animals were divided into three groups: Partial-width injury (PW), full-width injury (FW), and Sham (n=15-18 per group) with all animals undergoing to retroperitoneal surgical exposure of the lumbar IVD. The groups were cross-sectionally evaluated at 2-, 4-, and 8-week post-surgery time points. The injury was delivered to the L6/S1 IVD with the L5/6 IVD used as the internal control.
The partial-width injury mimics a localized injury to the annulus fibrosus, aka annular tear injury, in humans17. To evaluate the feasibility of this injury, we first performed the partial-width injury on six animals, and then they were euthanized shortly after recovery from anesthesia. The IVDs were harvested and measured for the thickness of the annulus fibrosus and the depth of the injury using contrast-enhanced microCT (CEμCT) and histological analysis.
The second set of animals were allowed to recover following PW, FW or Sham surgery for 2, 4 or 8 weeks (n = 5-6 per group) and then euthanized, and the IVD tissues were assessed for degeneration with CEμCT and histological analysis. Samples where attenuation was saturated were not included in analysis.
Mice were anesthetized with isoflurane gas in oxygen via a facemask (3–4% induction and 2–2.5% maintenance at 1 L/min flow rate; Highland Medical Equipment) and were given a preoperative intradermal injection of lidocaine (7 mg/kg; Hospira, Inc). The left flank was then shaved from the ventral to the dorsal midlines, and the skin was sterilized. The skin was prepared for aseptic surgery via washing with 70% ethanol and povidone iodine.
Partial-width injury
A distance of 0.3 mm from the end of the No. 11-scalpel blade tip was measured and marked with a micro-caliper under a microscope (Figure 1). The distance of 0.3 mm was determined from our preliminary studies. The edge of the blade was allowed to insert into the IVD until the 0.3 mm marking was no long visible under the microscope. Once pierced, the injury site was closely observed under the microscope to confirm that there is no leakage of the NP.
Full-width injury
A 33G needle was inserted bilaterally through the IVD lateral axis of the IVD. In contrast to the partial-width injury, NP herniations were observed following the full-width injury.
Contrast-enhanced microCT tomography (CEμCT)
Samples were incubated in a solution of 175 mg/mL solution diluted from a stock of OptiRay 350 (Guerbet, St. Louis) in PBS at 37°C. After 24 hrs of incubation, samples were scanned using a μCT40 (Scanco Medical, CH) at a 10-μm voxel size (45 kVp, 177 uA, high resolution, 300 ms integration).
CEμCT data was exported as a DICOM file for analysis in a custom MATLAB program. After an initial median filter (sigma = 0.8, support = 5), functional spine units were isolated from surrounding soft tissue not part of the IVD by drawing a contour around the outer edge every 5 transverse slices and morphing using linear interpolation. The IVD was manually segmented from the vertebral bodies with the same methodology as above. The remaining voxels were designated as the whole disc mask. The NP was thresholded from the AF followed by a morphological close and morphological open to fill interior holes and smooth the boundary. The volumes and average attenuations (intensity) were calculated from the mask of the NP and whole disc. The volume was determined from the total number of voxels contained within the mask and the attenuation was taken as the average 16-bit grayscale value of the voxels. Visualizations of the microCT were obtained using the image processing application OsiriX (Pixmeo, Geneva). AF thickness, partial-width injury depth and disc height index (DHI) were measured along the mid-sagittal plane. DHI was calculated as the ratio of the IVD height to width. IVD height was taken as the average at 5 equidistant points along the mid-sagittal plane. The ratio of NP intensity/disc intensity (NI/DI), defined as the average attenuation of voxels in the NP mask divided by the full disc mask, is an unbiased, fully three-dimensional measure that quantifies the relative size and hydration to inform the relative changes in degeneration 18.
Histological analysis
Following microCT, samples were fixed for 24 hours in 10% neutral buffered formalin followed by 3 days of decalcification in Immunocal (StatLab 1414-X). The samples were embedded in paraffin, sectioned at a thickness of 10 μm, and then stained with Safranin-O and Fast Green.
Measuring thickness of AF and the depth of partial-width injury
The CEμCT of the injured L6/S1 and histological analysis on uninjured L5/6 were used to measure the thickness of the AF. The CEμCT on the injured L6/S1 was used to measure the depth of injury as defined by the shortest perpendicular distance from the outer edge of annulus fibrosus to the visually observable outline of the injury site.
Assessment of IVD degeneration
Histological classification system recently developed by Melgoza et al. in 2021 was used to quantify the degeneration of the injured level (L6/S1) which allows the independent evaluation of the nucleus pulposus, annulus fibrosus, endplates and interface boundaries19. Morphology and NI/DI determined from CEμCT was used to further inform the level of degeneration of the injured level (L6/S1) compared to the internal control (L5/6).
Results
A total of 57 mice were subjected to the surgical procedure. The average surgery time was 15 min 38 sec ± 6 min 23 sec from incision to closure. There was no mortality or severe complication such as massive bleeding due to vascular injury during surgery. Caution was taken to prevent injury of the lumbosacral plexus located posteriorly during the blunt dissection of the psoas muscle from posterior to the anterior direction.
Annulus Fibrosus (AF) Thickness
CEμCT and histology measurements of AF thickness and IVD width were highly consistent. The anteroposterior IVD width measured with CEμCT was 1.27 ± 0.13 mm (mean ± standard deviation), whereas that measured by histological analysis was 1.21 ± 0.11 mm. AF thickness measured with CEμCT was 0.38±0.05 mm and that measured with histological analysis was 0.43 ± 0.10 mm (Figure 3). The CEμCT measured values were statistically indistinguishable from (p = 0.37) and were highly correlated (r2 = 0.96) with the histologically measured values (Figure 2).
Depth of Partial-width Injury
All injuries except one were isolated to the AF (Figure 3B). In sample 5, the NP may have been injured because CEμCT showed disruption of the AF/NP boundary (Figure 3C). The depth of injury measured with CEμCT was 0.29 ± 0.05 mm. The ratio of the injury depth to the AF thickness was 0.80 ± 0.19 (Figure 3D and 3E).
Structural evaluation of the IVD
Neither the partial-width nor the full-width injuries resulted in changes in the Disc Height Index across the two-, four-, and eight-week time points (Figure 4D). Similarly, the size of the nucleus pulposus, characterized by the nucleus pulposus (NP) volume fraction, were not dramatically different between groups and time points (Figure 4E). The relative hydration of the NP, quantified by the ratio of NP attenuating intensity to that of the whole disc (NI/DI), revealed the full-width injury caused a dramatic decrease in the attenuation of the NP at the two- and four-week time points, with a trending change at eight-weeks. Reduced NI/DI indicates a loss of NP hydration.
The impact of the partial- and full-width injuries to the annulus fibrosus was observable at two weeks, The relative hydration of the annulus fibrosus (AF) increases after an injury, and this is evident in both the partial- and full-width groups. Two weeks after injury, both the partial- and full-injuries showed an increase attenuation of the AF, with the full-width injury group sustaining this increase (Figure 4G).
Extent of IVD Degeneration
Quantitative histological analyses revealed a differential degenerative cascade to the IVD following full-width injury as early as two weeks post injury but not with the partial-width injury. The histological classification showed significant degeneration following FW injury at all timepoints (p < 0.05) in the NP, AF, interface boundaries, and total IVD score but not endplate score compared to Sham while no differences were detected between PW and Sham (Figure 5). No morphological differences between timepoints or injury groups were observed.
Discussion
Animal models of IVD degeneration are imperative to elucidate the key molecular mechanisms of the pathophysiology. To date, rodents, rabbits, dogs, goats, sheep, and primates have been used as models for intervertebral disc degeneration20. One main advantage of mouse models is the availability of reagents and modifications that could be used concomitantly with surgically induced IVD degeneration. However, the small size of the mouse requires a high degree of surgical precision, particularly with access and exposure of the lumbar spine. While many studies utilize the mouse tail for degenerative models12,21–23, the lumbar spine maintains the anatomical proximity to physiologically relevant structures such as the dorsal root ganglions and may better recapitulate the human disease22.
We describe here a novel procedure for mouse lumbosacral IVD injury with visual guidance via microscopy and gross features. The retroperitoneal space of the spine can be located (Figure S1) with the left pelvic bone with gluteus muscles and proximal thigh as landmarks (Figure 1C and 1D). Blunt-dissection of the fat pad surrounding the thoracic cage anteriorly, the gluteus muscles superiorly, and the thigh muscles posteriorly (Figure 1B) exposes these two landmarks (Figure 1C). The lateral approach to the IVD allows for precise injury to the annulus fibrosus of the IVD (Figure 1). Exposure of the pelvic bone from the gluteus muscle and rotation of the pelvic bone posteriorly is necessary to achieve ample exposure of multiple IVDs in the surgical field. The psoas muscles can be easily scraped posterior-anteriorly with a Penfield dissector. CEμCT allowed for a 3D spatially unbiased quantification of morphology and composition18 that confirmed the localization of injury across the cross-section time points. CEμCT measurements of the IVD morphology were in excellent agreement with histological measures. NI/DI and histological analysis indicated degeneration of injured IVDs starting 4 weeks post-surgery.
The present study describes a surgical procedure to expose the lumbosacral spine of mice and to induce a localized experimental injury with AF-limited depth and compared the degenerative response with a more commonly used needle puncture injury. The technique described herein also focused on achieving a consistent depth and size of injury. The marked tip of the No. 11-scalpel blade tip was confirmed with CEμCT to provide AF-limited injury and resulted in no NP leakage under observation by microscopy. The various sizes of the needle may produce inconsistent depth and size of injury. A thick needle may lead to end-plate injury due to the small IVD height in mice, whereas the excessive flexibility of a thin needle would contribute to inconsistency in the depth of injury. While most injury models cause damage to the nucleus pulposus, the cartilaginous endplates, and even the vertebral body24–26, the partial-width injury here results in an isolated injury to the outer annulus fibrosus. Surprisingly, we observed no degenerative changes in the endplate in the full-width injury, suggesting that it is possible to induce an IVD only injury using a carefully applied 33G needle. Following full-width injury, IVDs exhibited quick and sustained degeneration as early as two weeks after injury, and this is sustained through the four- and eight-week time points. Despite maintaining disc height, the FW injury caused consistent degenerative changes in the nucleus pulposus, annulus fibrosus, and interface boundaries which included the AF-endplate and NP-AF boundaries. The PW injury did not produce statistically significant degenerative changes, but whether the innervation or vascularization profile changes particularly at the outer annulus fibrosus remains to be investigated.
CEμCT allowed for visualization of the injury site following both partial- and full-width injuries. The increased attenuation on CEμCT may indicate changes in composition or diffusion properties resulting from the disruption in the annulus fibrosus with both injuries. The injured annulus fibrosus appears to be highly hydrated, due to the increased interstitial fluids that localize around the injury site (Figures 3–4). Since IVD degeneration in humans often starts as a tear in the annulus fibrosus 17, this model will enable mechanistic investigations of how injuries of the annulus fibrosus contribute to IVD degeneration and the subsequent development of low back pain. In contrast, the full-width injury to the nucleus pulposus induces a more rapid and severe course of degeneration that is more aligned with an acute trauma 7,20,21. Unlike the increased attenuation of the injured annulus fibrosus, the depressurized nucleus pulposus loses water and attenuates less than the healthy state (Figures 4). Consistent with unilateral injuries of the tail IVD, the resultant degenerative cascade was nuanced11, and required high resolutions modalities to detect measurable changes. Future studies will evaluate the partial-width injury as a slowly progressing IVD degeneration model following a clinically relevant injury.