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Contrast-enhanced microCT evaluation of degeneration following partial and full width injuries to mouse lumbar intervertebral disc

Remy E. Walk, Hong Joo Moon, View ORCID ProfileSimon Y. Tang, Munish C. Gupta
doi: https://doi.org/10.1101/2022.01.14.476362
Remy E. Walk
1Dept of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA
2Dept of Orthopaedic Surgery, Washington University in St. Louis, St. Louis, MO, USA
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Hong Joo Moon
2Dept of Orthopaedic Surgery, Washington University in St. Louis, St. Louis, MO, USA
3Dept of Neurosurgery, Korea University College of Medicine, Seoul, South Korea
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Simon Y. Tang
1Dept of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA
2Dept of Orthopaedic Surgery, Washington University in St. Louis, St. Louis, MO, USA
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  • For correspondence: simon.tang@wustl.edu
Munish C. Gupta
2Dept of Orthopaedic Surgery, Washington University in St. Louis, St. Louis, MO, USA
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Abstract

Study Design Preclinical animal study

Objective Evaluation of the degenerative progression resulting from either a partial- or full-width injury to the mouse lumbar intervertebral disc (IVD) using contrast-enhanced micro-computed tomography and histological analyses. We utilized a lateral-retroperitoneal surgical approach to access the lumbar IVD, and the injuries to the IVD were induced by either incising one side of the annulus fibrosus or puncturing both sides of the annulus fibrosus. The full-width injury caused dramatic reduction in nucleus pulposus hydration and significant degeneration. A partial-width injury produces localized deterioration around the annulus fibrosus site that resulted in local tissue remodeling without gross degeneration to the IVD.

Methods Female C57BL/6J mice of 3-4 months age were used in this study. They were divided into three groups to undergo a partial-width, full-width, or sham injuries. The L5/L6 and L6/S1 lumbar IVDs were surgically exposed using a lateral-retroperitoneal approach. The L6/S1 IVDs were injured using either a surgical scalpel (partial-width) or a 33G needle (full-width), with the L5/L6 serving as an internal control. These animals were allowed to recover and then sacrificed at 2-, 4-, or 8-weeks post-surgery. The IVDs were assessed for degeneration using contrast-enhanced microCT (CEµCT) and histological analysis.

Results The high-resolution 3D evaluation of the IVD confirmed that the respective injuries localized within one side of the annulus fibrosus or spanned the full width of the IVD. The full-width injury caused deteriorations in the nucleus pulposus after 2 weeks that culminated in significant degeneration at 8 weeks, while the partial width injury caused localized disruptions that remained limited to the annulus fibrosus.

Conclusion The use of CEμCT revealed distinct IVD degeneration profiles resulting from partial- and full-width injuries. The partial width injury may serve as a better model for IVD degeneration resulting from localized annulus fibrosus injuries in humans.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted January 16, 2022.
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Contrast-enhanced microCT evaluation of degeneration following partial and full width injuries to mouse lumbar intervertebral disc
Remy E. Walk, Hong Joo Moon, Simon Y. Tang, Munish C. Gupta
bioRxiv 2022.01.14.476362; doi: https://doi.org/10.1101/2022.01.14.476362
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Contrast-enhanced microCT evaluation of degeneration following partial and full width injuries to mouse lumbar intervertebral disc
Remy E. Walk, Hong Joo Moon, Simon Y. Tang, Munish C. Gupta
bioRxiv 2022.01.14.476362; doi: https://doi.org/10.1101/2022.01.14.476362

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