Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Assessing Myf5 and Lbx1 contribution to carapace development by reproducing their turtle-specific signatures in mouse embryos

Triin Tekko, Ana Nóvoa, View ORCID ProfileMoisés Mallo
doi: https://doi.org/10.1101/2022.01.18.476730
Triin Tekko
1Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ana Nóvoa
1Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Moisés Mallo
1Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Moisés Mallo
  • For correspondence: mallo@igc.gulbenkian.pt
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Background The turtle carapace is an evolutionary novelty resulting from changes in the processes that build ribs and their associated muscles in most tetrapod species. Turtle embryos have several unique features that might play a role in this process, including the carapacial ridge, a Myf5 gene with shorter coding region that generates an alternative splice variant lacking exon 2, and unusual expression patterns of Lbx1 and HGF.

Results We generated Myf5 alleles reproducing the Myf5 turtle expression features. At mid gestation, mouse embryos expressing Myf5 lacking exon 2 reproduced some early properties of turtle somites, but still developed into viable and fertile mice. Extending Lbx1 expression into the hypaxial dermomyotomal lip of trunk somites to mimic the turtle Lbx1 expression pattern, produced fusions in the distal part of the ribs.

Conclusions Turtle-like Myf5 activity might generate a plastic state in developing trunk somites under which they can either enter carapace morphogenetic routes, possibly triggered by signals from the carapacial ridge, or still engage in the development of a standard tetrapod ribcage in the absence of those signals. In addition, trunk Lbx1 expression might play a later role in the formation of the lateral border of the carapace.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Funding information: PTDC/BIA-BID/30254/2017, from Fundação para a Ciência e a Tecnologia, Portugal. Congento, project LISBOA-01-0145-FEDER-022170 from Fundação para a Ciência e a Tecnologia, Portugal.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted January 18, 2022.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Assessing Myf5 and Lbx1 contribution to carapace development by reproducing their turtle-specific signatures in mouse embryos
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Assessing Myf5 and Lbx1 contribution to carapace development by reproducing their turtle-specific signatures in mouse embryos
Triin Tekko, Ana Nóvoa, Moisés Mallo
bioRxiv 2022.01.18.476730; doi: https://doi.org/10.1101/2022.01.18.476730
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Assessing Myf5 and Lbx1 contribution to carapace development by reproducing their turtle-specific signatures in mouse embryos
Triin Tekko, Ana Nóvoa, Moisés Mallo
bioRxiv 2022.01.18.476730; doi: https://doi.org/10.1101/2022.01.18.476730

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3609)
  • Biochemistry (7584)
  • Bioengineering (5533)
  • Bioinformatics (20816)
  • Biophysics (10341)
  • Cancer Biology (7992)
  • Cell Biology (11652)
  • Clinical Trials (138)
  • Developmental Biology (6616)
  • Ecology (10222)
  • Epidemiology (2065)
  • Evolutionary Biology (13639)
  • Genetics (9553)
  • Genomics (12856)
  • Immunology (7928)
  • Microbiology (19561)
  • Molecular Biology (7674)
  • Neuroscience (42165)
  • Paleontology (308)
  • Pathology (1259)
  • Pharmacology and Toxicology (2204)
  • Physiology (3271)
  • Plant Biology (7052)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1953)
  • Systems Biology (5431)
  • Zoology (1119)