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Mutations in TIC100 impair and repair chloroplast protein import and impact retrograde signalling

View ORCID ProfileNaresh Loudya, Douglas P. F. Maffei, View ORCID ProfileJocelyn Bédard, View ORCID ProfileSabri Mohd. Ali, View ORCID ProfilePaul Devlin, View ORCID ProfileR. Paul Jarvis, View ORCID ProfileEnrique López-Juez
doi: https://doi.org/10.1101/2022.01.18.476798
Naresh Loudya
aDepartment of Biological Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK
bDepartment of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK
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Douglas P. F. Maffei
aDepartment of Biological Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK
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Jocelyn Bédard
bDepartment of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK
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Sabri Mohd. Ali
bDepartment of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK
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Paul Devlin
aDepartment of Biological Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK
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R. Paul Jarvis
bDepartment of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK
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  • For correspondence: e.lopez@rhul.ac.uk paul.jarvis@plants.ox.ac.uk
Enrique López-Juez
aDepartment of Biological Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK
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  • For correspondence: e.lopez@rhul.ac.uk paul.jarvis@plants.ox.ac.uk
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Abstract

Chloroplast biogenesis requires synthesis of proteins in the nucleocytoplasm and the chloroplast itself. Nucleus-encoded chloroplast proteins are imported via multiprotein translocons in the organelle’s envelope membranes. Controversy exists around whether a 1 MDa complex comprising TIC20, TIC100 and other proteins constitutes the inner membrane TIC translocon. The Arabidopsis cue8 virescent mutant is broadly defective in plastid development. We identify CUE8 as TIC100. The tic100cue8 mutant accumulates reduced levels of 1 MDa complex components and exhibits reduced import of two nucleus-encoded chloroplast proteins of different import profiles. A search for suppressors of tic100cue8 identified a second mutation within the same gene, tic100soh1, which rescues the visible, 1 MDa complex-subunit abundance, and chloroplast protein import phenotypes. tic100soh1 retains but rapidly exits virescence, and rescues the synthetic lethality of tic100cue8 when retrograde signalling is impaired by the gun1 mutation. Alongside the strong virescence, changes in RNA editing and the presence of unimported precursor proteins show that a strong signalling response is triggered when TIC100 function is altered. Our results are consistent with a role for TIC100, and by extension the 1 MDa complex, in the chloroplast import of photosynthetic and non-photosynthetic proteins, a process which initiates retrograde signalling.

One sentence summary Complementary mutations in TIC100 of the chloroplast inner envelope membrane cause reductions or corrective improvements in chloroplast protein import, and highlight a signalling role.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted January 22, 2022.
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Mutations in TIC100 impair and repair chloroplast protein import and impact retrograde signalling
Naresh Loudya, Douglas P. F. Maffei, Jocelyn Bédard, Sabri Mohd. Ali, Paul Devlin, R. Paul Jarvis, Enrique López-Juez
bioRxiv 2022.01.18.476798; doi: https://doi.org/10.1101/2022.01.18.476798
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Mutations in TIC100 impair and repair chloroplast protein import and impact retrograde signalling
Naresh Loudya, Douglas P. F. Maffei, Jocelyn Bédard, Sabri Mohd. Ali, Paul Devlin, R. Paul Jarvis, Enrique López-Juez
bioRxiv 2022.01.18.476798; doi: https://doi.org/10.1101/2022.01.18.476798

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