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Sialic acid and fucose residues on the SARS-CoV-2 receptor binding domain modulate IgG reactivity

Ebba Samuelsson, Ekaterina Mirgorodskaya, Kristina Nyström, Malin Bäckström, Jan-Åke Liljeqvist, View ORCID ProfileRickard Nordén
doi: https://doi.org/10.1101/2022.01.20.477056
Ebba Samuelsson
1Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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Ekaterina Mirgorodskaya
2Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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Kristina Nyström
1Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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Malin Bäckström
3Mammalian Protein Expression Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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Jan-Åke Liljeqvist
1Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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Rickard Nordén
1Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
4Department of Clinical Microbiology, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden
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  • ORCID record for Rickard Nordén
  • For correspondence: rickard.norden@microbio.gu.se
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Abstract

The receptor binding domain (RBD) of the SARS-CoV-2 spike protein is a conserved domain and a target for neutralizing antibodies. We defined the carbohydrate content of recombinant RBD produced in different mammalian cells. We found a higher degree of complex type N-linked glycans, with less sialylation and more fucosylation, when the RBD was produced in Human embryonic kidney cells compared to the same protein produced in Chinese hamster ovary cells. The carbohydrates on the RBD proteins were enzymatically modulated and the effect on antibody reactivity was evaluated with serum samples from SARS-CoV-2 positive patients. Removal of all carbohydrates diminished antibody reactivity while removal of only sialic acids or terminal fucoses improved the reactivity. The RBD produced in Lec3.2.8.1-cells, which generate carbohydrate structures devoid of sialic acids and with reduced fucose content, exhibited enhanced antibody reactivity verifying the importance of these specific monosaccharides. The results can be of importance for the design of future vaccine candidates, indicating that it might be possible to enhance the immunogenicity of recombinant viral proteins.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 21, 2022.
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Sialic acid and fucose residues on the SARS-CoV-2 receptor binding domain modulate IgG reactivity
Ebba Samuelsson, Ekaterina Mirgorodskaya, Kristina Nyström, Malin Bäckström, Jan-Åke Liljeqvist, Rickard Nordén
bioRxiv 2022.01.20.477056; doi: https://doi.org/10.1101/2022.01.20.477056
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Sialic acid and fucose residues on the SARS-CoV-2 receptor binding domain modulate IgG reactivity
Ebba Samuelsson, Ekaterina Mirgorodskaya, Kristina Nyström, Malin Bäckström, Jan-Åke Liljeqvist, Rickard Nordén
bioRxiv 2022.01.20.477056; doi: https://doi.org/10.1101/2022.01.20.477056

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