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Dynamic observation of 2H labeled compounds in the human brain with 1H versus 2H magnetic resonance spectroscopy at 9.4T

View ORCID ProfileLoreen Ruhm, View ORCID ProfileTheresia Ziegs, Andrew Martin Wright, View ORCID ProfileClaudius Sebastian Mathy, View ORCID ProfileSaipavitra Murali-Manohar, View ORCID ProfileJohanna Dorst, View ORCID ProfileNikolai Avdievich, View ORCID ProfileAnke Henning
doi: https://doi.org/10.1101/2022.01.24.477582
Loreen Ruhm
1High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany
2IMPRS for Cognitive and Systems Neuroscience, Eberhard-Karls University of Tübingen, Germany
3Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas/Texas, USA
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  • For correspondence: Loreen.ruhm@tuebingen.mpg.de
Theresia Ziegs
1High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany
2IMPRS for Cognitive and Systems Neuroscience, Eberhard-Karls University of Tübingen, Germany
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Andrew Martin Wright
1High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany
2IMPRS for Cognitive and Systems Neuroscience, Eberhard-Karls University of Tübingen, Germany
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Claudius Sebastian Mathy
4Department of Radiology and Biomedical Imaging, Magnetic Resonance Research Center, Yale University, New Haven, CT, USA
5Institute of Radiology, Friedrich-Alexander University of Erlangen-Nurnberg, Erlangen, Germany
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Saipavitra Murali-Manohar
1High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany
6F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, Maryland, USA
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Johanna Dorst
1High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany
2IMPRS for Cognitive and Systems Neuroscience, Eberhard-Karls University of Tübingen, Germany
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Nikolai Avdievich
1High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany
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Anke Henning
1High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany
3Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas/Texas, USA
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Abstract

The metabolic pathway of [6,6’-2H2]-labeled glucose was investigated with two different techniques. The first technique used direct detection of deuterium applying Deuterium Metabolic Imaging (DMI). The second technique used the indirect detection of deuterium with proton MR spectroscopy (MRS) called Quantitative Exchanged-label Turnover (QELT) MRS. For the first time, time-resolved data was acquired for both techniques in the same healthy human subjects and directly compared. The time-curves were used in a kinetic model to estimate rates of the metabolic pathway of glucose. Two different kinetic models were compared. One included only DMI data, the second one combined DMI and QELT. For the first model, a tricarboxylic acid (TCA) cycle rate of 0.69 ± 0.10 μmol·min-1·g-1 was determined. For the second model, the estimated TCA cycle rate was 0.68 ± 0.12 μmol·min-1·g-1. In addition, the rate of glutamine synthesis from glutamate could be estimated with model 2 (0.51 ± 0.15 μmol·min-1·g-1). The sensitivity of both methods was evaluated and compared to alternative techniques.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 27, 2022.
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Dynamic observation of 2H labeled compounds in the human brain with 1H versus 2H magnetic resonance spectroscopy at 9.4T
Loreen Ruhm, Theresia Ziegs, Andrew Martin Wright, Claudius Sebastian Mathy, Saipavitra Murali-Manohar, Johanna Dorst, Nikolai Avdievich, Anke Henning
bioRxiv 2022.01.24.477582; doi: https://doi.org/10.1101/2022.01.24.477582
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Dynamic observation of 2H labeled compounds in the human brain with 1H versus 2H magnetic resonance spectroscopy at 9.4T
Loreen Ruhm, Theresia Ziegs, Andrew Martin Wright, Claudius Sebastian Mathy, Saipavitra Murali-Manohar, Johanna Dorst, Nikolai Avdievich, Anke Henning
bioRxiv 2022.01.24.477582; doi: https://doi.org/10.1101/2022.01.24.477582

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