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Selective Ablation of 3’ RNA ends and Processive RTs Facilitate Direct cDNA Sequencing of Full-length Host Cell and Viral Transcripts
View ORCID ProfileChristian M. Gallardo, Anh-Viet T. Nguyen, View ORCID ProfileAndrew L. Routh, View ORCID ProfileBruce E. Torbett
doi: https://doi.org/10.1101/2022.01.27.478099
Christian M. Gallardo
1Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA
2Center for Immunity and Immunotherapies, Seattle Children’s Research Institute, Seattle WA, USA
Anh-Viet T. Nguyen
1Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA
Andrew L. Routh
3Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX, USA
4Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX, USA
Bruce E. Torbett
1Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA
2Center for Immunity and Immunotherapies, Seattle Children’s Research Institute, Seattle WA, USA
5Institute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, USA
6Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, WA, USA
7Department of Pediatrics, University of Washington School of Medicine, Seattle, WA, USA

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Posted May 04, 2022.
Selective Ablation of 3’ RNA ends and Processive RTs Facilitate Direct cDNA Sequencing of Full-length Host Cell and Viral Transcripts
Christian M. Gallardo, Anh-Viet T. Nguyen, Andrew L. Routh, Bruce E. Torbett
bioRxiv 2022.01.27.478099; doi: https://doi.org/10.1101/2022.01.27.478099
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