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CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures

Anna I. Wurz, View ORCID ProfileWyatt Paul Bunner, View ORCID ProfileErzsebet M. Szatmari, View ORCID ProfileRobert M. Hughes
doi: https://doi.org/10.1101/2022.01.28.478241
Anna I. Wurz
†Department of Chemistry, East Carolina University, Greenville, North Carolina, United States
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Wyatt Paul Bunner
‡Department of Physical Therapy, East Carolina University, Greenville, North Carolina, United States
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Erzsebet M. Szatmari
‡Department of Physical Therapy, East Carolina University, Greenville, North Carolina, United States
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Robert M. Hughes
†Department of Chemistry, East Carolina University, Greenville, North Carolina, United States
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  • For correspondence: hughesr16@ecu.edu
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Abstract

BAR (Bin, Amphiphysin and Rvs) protein domains are responsible for the generation of membrane curvature and represent a critical mechanical component of cellular functions. Thus, BAR domains have great potential as components of membrane-remodeling tools for cell biologists. In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’) with applications in the remodeling of membrane architectures and the control of cellular dynamics. By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions. Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity. Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function. Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵* Contact: hughesr16{at}ecu.edu

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted January 28, 2022.
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CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures
Anna I. Wurz, Wyatt Paul Bunner, Erzsebet M. Szatmari, Robert M. Hughes
bioRxiv 2022.01.28.478241; doi: https://doi.org/10.1101/2022.01.28.478241
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CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures
Anna I. Wurz, Wyatt Paul Bunner, Erzsebet M. Szatmari, Robert M. Hughes
bioRxiv 2022.01.28.478241; doi: https://doi.org/10.1101/2022.01.28.478241

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