Abstract
We describe a new mouse model of rectal cancer (RC) involving rapid tumor organoid engraftment via orthotopic transplantation; the resulting RC tumors invaded inward from the mucosal surface and metastasized to distant organs. Histologically the tumors closely resemble human RC and mirror remodeling of the tumor microenvirnoment (TME) in response to radiation. This murine RC model thus recapitulates the pathogenesis of human RC, thereby fulfilling the need for a physiologically accurate model for preclinical efficacy studies.
Competing Interest Statement
P.B.R. reports prior research funding from EMD Serono. J.J.S. received travel support from Intuitive Surgical Inc. (2015) and served as a clinical advisor for Guardant Health Inc. (2019). C.L.S. serves on the Board of Directors of Novartis, is a co-founder of ORIC Pharmaceuticals and co-inventor of enzalutamide and apalutamide. He is a science advisor to Agios, Arsenal, Beigene, Blueprint, Column Group, Foghorn, Housey Pharma, Nextech, KSQ and PMV .
Footnotes
Disclosures: P.B.R. reports prior research funding from EMD Serono. J.J.S. received travel support from Intuitive Surgical Inc. (2015) and served as a clinical advisor for Guardant Health Inc. (2019). C.L.S. serves on the Board of Directors of Novartis, is a co-founder of ORIC Pharmaceuticals and co-inventor of enzalutamide and apalutamide. He is a science advisor to Agios, Arsenal, Beigene, Blueprint, Column Group, Foghorn, Housey Pharma, Nextech, KSQ and PMV.
Support: This work was supported in part by National Institutes of Health/National Cancer Institute (NIH/NCI) Memorial Sloan Kettering Cancer Center (MSK) Support Grant (P30 CA008748). J.K.K. is also supported by an ASCRS Grant (GSSRIG-044). Y.G. is supported by Sigrid Jusélius Fellowship Grant. J.J.S., C.W., J.S. are supported by NIH/NCI grant (R37 CA248289). J.J.S. and P.B.R. are supported by Memorial Sloan Kettering Cancer Center Geoffrey Beene Cancer Research Grant. P.B.R. is also supported by an NIH/NCI grant (K08CA255574), the Memorial Sloan Kettering Cancer Center Imaging and Radiation Sciences Program, and an NIH Loan Repayment Program (LRP) award.