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Synthetic memory circuits for programmable cell reconfiguration in plants

View ORCID ProfileJames P B Lloyd, Florence Ly, Patrick Gong, Jahnvi Pflüger, View ORCID ProfileTessa Swain, View ORCID ProfileChristian Pflüger, Muhammad Adil Khan, View ORCID ProfileBrendan Kidd, View ORCID ProfileRyan Lister
doi: https://doi.org/10.1101/2022.02.11.480167
James P B Lloyd
1Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
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Florence Ly
1Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
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Patrick Gong
1Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
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Jahnvi Pflüger
2Harry Perkins Institute of Medical Research, The University of Western Australia
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Tessa Swain
2Harry Perkins Institute of Medical Research, The University of Western Australia
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Christian Pflüger
1Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
2Harry Perkins Institute of Medical Research, The University of Western Australia
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Muhammad Adil Khan
1Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
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Brendan Kidd
1Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
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Ryan Lister
1Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia
2Harry Perkins Institute of Medical Research, The University of Western Australia
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  • For correspondence: ryan.lister@uwa.edu.au
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Abstract

Plant biotechnology predominantly relies on a restricted set of genetic parts with limited capability to customize spatiotemporal and conditional expression patterns. Synthetic gene circuits have the ability to integrate multiple customizable input signals through a processing unit constructed from biological parts, to produce a predictable and programmable output. Here, we present a suite of functional recombinase-based gene circuits for use in plants. We first established a range of key gene circuit components compatible with plant cell functionality. We then used these to develop a range of operational logic gates using the identify function (activation) and negation function (repression) in Arabidopsis protoplasts and in vivo, demonstrating their utility for programmable manipulation of transcriptional activity in a complex multicellular organism. Through utilization of genetic recombination these circuits create stable long-term changes in expression and recording of past stimuli. This highly-compact programmable gene circuit platform provides new capabilities for engineering sophisticated transcriptional programs and previously unrealised traits into plants.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 12, 2022.
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Synthetic memory circuits for programmable cell reconfiguration in plants
James P B Lloyd, Florence Ly, Patrick Gong, Jahnvi Pflüger, Tessa Swain, Christian Pflüger, Muhammad Adil Khan, Brendan Kidd, Ryan Lister
bioRxiv 2022.02.11.480167; doi: https://doi.org/10.1101/2022.02.11.480167
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Synthetic memory circuits for programmable cell reconfiguration in plants
James P B Lloyd, Florence Ly, Patrick Gong, Jahnvi Pflüger, Tessa Swain, Christian Pflüger, Muhammad Adil Khan, Brendan Kidd, Ryan Lister
bioRxiv 2022.02.11.480167; doi: https://doi.org/10.1101/2022.02.11.480167

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