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Nisin G is a novel nisin variant produced by a gut-derived Streptococcus salivarius

Garreth W. Lawrence, Enriqueta Garcia-Gutierrez, Calum J. Walsh, Paula M. O’Connor, Máire Begley, Paul D. Cotter, Caitriona M. Guinane
doi: https://doi.org/10.1101/2022.02.15.480493
Garreth W. Lawrence
aDepartment of Biological Sciences, Munster Technological University, Cork, T12 P928, Ireland
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Enriqueta Garcia-Gutierrez
bTeagasc Food Research Centre, Moorepark, Fermoy, Cork, P61 C996, Ireland
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Calum J. Walsh
bTeagasc Food Research Centre, Moorepark, Fermoy, Cork, P61 C996, Ireland
cAPC Microbiome Ireland, Cork, T12 YN60, Ireland
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Paula M. O’Connor
bTeagasc Food Research Centre, Moorepark, Fermoy, Cork, P61 C996, Ireland
cAPC Microbiome Ireland, Cork, T12 YN60, Ireland
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Máire Begley
aDepartment of Biological Sciences, Munster Technological University, Cork, T12 P928, Ireland
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Paul D. Cotter
bTeagasc Food Research Centre, Moorepark, Fermoy, Cork, P61 C996, Ireland
cAPC Microbiome Ireland, Cork, T12 YN60, Ireland
dVistaMilk SFI Research Centre, Moorepark, Fermoy, Cork, P61 C996, Ireland
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  • For correspondence: caitriona.guinane@mtu.ie paul.cotter@teagasc.ie
Caitriona M. Guinane
aDepartment of Biological Sciences, Munster Technological University, Cork, T12 P928, Ireland
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  • For correspondence: caitriona.guinane@mtu.ie paul.cotter@teagasc.ie
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Abstract

Fusobacterium nucleatum is an emerging human pathogen associated with a number of intestinal conditions including colorectal cancer (CRC) development. Screening for gut-derived strains that exhibit anti-F. nucleatum activity revealed Streptococcus salivarius DPC6487 as a strain of interest. Whole genome sequencing analysis of DPC6487 resulted in the identification of a gene predicted to encode a novel nisin variant designated nisin G. The structural nisin G peptide differs from the prototypical nisin A with respect to seven amino acids (Ile4Tyr, Ala15Val, Gly18Ala, Asn20His, Met21Leu, His27Asn and His31Ile), including differences that have not previously been associated with a natural nisin variant. The nisin G gene cluster consists of nsgGEFABTCPRK with transposases encoded between the nisin G structural gene (nsgA) and nsgF. The cluster lacked an equivalent to the nisI immunity determinant. S. salivarius DPC6487 exhibited a narrower spectrum of activity compared to the nisin A producer, Lactococcus lactis NZ9700, when assessed through deferred antagonism-based assays. Such narrow spectrum activity is desirable as it is less likely to lead to collateral damage to gut commensals.

Ultimately, this is the first report of a nisin variant produced by a representative of a species that is frequently a focus for probiotic development. The production of this bacteriocin by a gut-derived S. salivarius and its narrow spectrum activity against F. nucleatum indicates that this strain merits further attention to determine its potential for probiotic-related applications.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted February 15, 2022.
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Nisin G is a novel nisin variant produced by a gut-derived Streptococcus salivarius
Garreth W. Lawrence, Enriqueta Garcia-Gutierrez, Calum J. Walsh, Paula M. O’Connor, Máire Begley, Paul D. Cotter, Caitriona M. Guinane
bioRxiv 2022.02.15.480493; doi: https://doi.org/10.1101/2022.02.15.480493
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Nisin G is a novel nisin variant produced by a gut-derived Streptococcus salivarius
Garreth W. Lawrence, Enriqueta Garcia-Gutierrez, Calum J. Walsh, Paula M. O’Connor, Máire Begley, Paul D. Cotter, Caitriona M. Guinane
bioRxiv 2022.02.15.480493; doi: https://doi.org/10.1101/2022.02.15.480493

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